Determination of Some Morphological Traits and Genetic Factor of Two Species of Liriomyza spp (Diptera: Agromyzidae) on the Climbing Beans and Tomato Crops (original) (raw)

This study was carried out to study some morphological parts of adult male of Liriomyza spp, including the antenna, the femur and the middle vein (Cu Al), as well as the shape of mines that larvae made them on the leaves. Results showed that there were two species of L.bryoinae on tomato crop and L.sativae on climbing beans crop, this was agreed with the molecular study that it was conducted using the RAPD Random Amplification of Polymorphic DNA based on the PCR technique to determine genetic variability of DNA that it was extraction from adults and pupae and obtained a quantity of it ranged from (35-556.9) mg and purity ranged between (1.7-2). The RAPD markers were studied using 19 random primers, 13 of which showed a bands and 6 of them didn’t show any bands, the total number of bands loci were 53 loci, 23 of which were main bands and 30 were polymorphism and genetic analysis was performed based on these results. The genetic distance was found and lowest was (0...