In Vitro Perfected Propagation Biotechnology of Prunus Serrulata Species (original) (raw)
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Plant Cell Tissue and Organ Culture, 2005
In vitro culture establishment, shoot proliferation and ex vitro rooting responses of Mongolian cherry (Prunus fruticosa L.), and Nanking cherry (Prunus tomentosa L.), were examined using various combinations of growth regulators. Dormant buds, taken during winter months, were used as explants. In both species, Murashige and Skoog Minimal Organic (MSMO) solid medium supplemented with 0.49 μM indole-3-butyric acid (IBA) and either 4.44 or 8.88 μM 6-benzylaminopurine (BA), was the best for culture initiation, and with 8.88–15.16 μM BA for shoot proliferation. Good rooting responses were also obtained with shoots produced on media containing 0.91 μM thidiazuron (TDZ). Auxin treatments were required for ex vitro rooting of approximately 20 mm long shoots in peat/perlite (1:1 v/v) mixture, at 25 °C, under mist. The best rooting (79%) was obtained with IBA/NAA (naphthaleneacetic acid) (9.80/2.69 μM) combination. A commercial rooting powder, Rootone F, containing IBA/NAA (0.057/0.067%), was also effective (73%). The ex vitro rooted plantlets did not require any additional acclimatization prior to transplanting to the regular greenhouse conditions.
Optimization of Nutrient Media of in Vitro Propagation for Some Cherry Rootstocks (Prunus Cerasus)
ANGLISTICUM. Journal of the Association for Anglo-American Studies, 2018
The most important species of which have origin many populations, land-races and local varieties: wild cherry, wild pears, wild almond, wild medlar, etc. This study was focused in many important rootstocks of P. avium x P canascens - Gisela 6, P.avium x P. pseudocerasus - Colt (nano), P. mahaleb x P.avium (Maxma), P. Mahaleb and cultivar Prunus avium L. (Cerasus avium L. var. sativa Ser., P. cerasus): Bigarreau Hatif. Are used two important media in the micro propagation of the horticultural species above, MS (Murashige & Skoog) and LP (Quoirin & Lepoivre). Nutrients of MS media have affected with sensible changes compare with LP media, in the explants proliferation for differentiations of the buds, shoots and leafs. Inoculation of explants in LP media, results with lower result on the qualitative indicators of the research. Average growing of the shoots on the MS media, resulted 10.1 mm while on the LP media was 6.0 mm. Average number of leafs 2.47 on the MS media, while on the med...
Adventitious Shoot Regeneration and Rooting of Prunus serotina In Vitro
A complete regeneration protocol was developed for Prunus serotina Ehrh., an important hardwood species for timber and sawlog production in the central and eastern United States. Nodal sections were cultured on Murashige and Skoog (MS) medium supple- mented with 4.44 µM 6-benzylaminopurine (BA), 0.49 µM indole-3-butyric acid (IBA), and 0.29 µM gibberellic acid (GA3). In vitro leaf explants of three genotypes were placed on woody plant medium (WPM) supplemented with 0, 2.27, 4.54, or 6.81 µM thidiazuron (TDZ) in combination with 0, 0.54, 1.07, or 5.37 µM naphthaleneacetic acid (NAA), and on WPM supplemented with 0, 4.44, 8.88, or 13.32 µM BA in combination with 0, 0.54, 1.07, or 5.37 µM NAA. Cultures were maintained either in continuous darkness for 5 weeks, or in the dark for 3 weeks and then transferred to a 16-hour photoperiod. TDZ and the genotype had a signifi cant effect on the number of shoots regenerated. The maximum mean number of shoots regenerated per explant (5.05 ± 1.14)...
2011
PHL-A is a hybrid of Prunus avium × Prunus cerasus. Growth reduction reaches 70 percent for this rootstock, in comparison with seedling cherry rootstocks, resulting in higher harvest with lower production costs. In this research, the effects of different culture media and plant growth regulators in micropropagation of this rootstock were investigated. Shoot tips and axillary buds, derived from greenhouse-grown one-year-old trees, were used as explants and established in vitro. In proliferation stage, multiplication rate of about 5.37 was achieved over a 4 week period using MS medium with 0.5 mg l-1 benzyladenine. Rooting of shoots, with up to 100 percent efficiency, were also obtained within four weeks on DKW medium without growth regulators in vitro. Application of double-phase medium instead of agar-gelled medium resulted in better control of hyperhydricity. In the acclimatization stage, rooted plantlets were transferred to peatmoss: cocopeat: perlite mixture (2:2:1 v/v/v) that we...