Aloe ferox Research Papers - Academia.edu (original) (raw)

Background: Although Aloe vera extracts have exhibited various effects on the skin, less is known about the biological activities of other aloe species on the skin. Objective: To investigate the skin hydrating and anti-erythema... more

Background: Although Aloe vera extracts have exhibited various effects on the skin, less is known about the biological activities of other aloe species on the skin. Objective: To investigate the skin hydrating and anti-erythema activity of gel materials from Aloe marlothii A. Berger and A. ferox Mill. in comparison to that of Aloe barbadensis Miller (Aloe vera) in healthy human volunteers. Methods: Aqueous solutions of the polisaccharidic fractions of the selected aloe leaf gel materials were applied to the volar forearm skin of female subjects. The hydration effect of the aloe gel materials were measured with a Corneometer® CM 825, Visioscan® VC 98 and Cutometer® dual MPA 580 after single and multiple applications. The Mexameter® MX 18 was used to determine the anti-erythema effects of the aloe material solutions on irritated skin areas. Results: The A. vera and A. marlothii gel materials hydrated the skin after a single application, whereas the A. ferox gel material showed dehydration effects compared to the placebo. After multiple applications all the aloe materials exhibited dehydration effects on the skin. Mexameter® readings showed that A. vera and A. ferox have anti-erythema activity similar to that of the positive control group (i.e. hydrocortisone gel) after 6 days of treatment. Conclusion: The polysaccharide component of the gel materials from selected aloe species has a dehydrating effect on the skin after multiple applications. Both A. vera and A. ferox gel materials showed potential to reduce erythema on the skin similar to that of hydrocortisone gel.

Aim of the study: The present study aimed at determining the wound healing properties of the gel and whole-leaf materials of Aloe vera, Aloe ferox and Aloe marlothii, as well as their cytotoxic effects on normal human keratinocyte cells... more

Aim of the study: The present study aimed at determining the wound healing properties of the gel and whole-leaf materials of Aloe vera, Aloe ferox and Aloe marlothii, as well as their cytotoxic effects on normal human keratinocyte cells (HaCaT).
Materials and Methods: Nuclear magnetic resonance spectroscopy was used to chemically fingerprint the aloe gel and whole-leaf materials by identifying characteristic marker molecules of aloe gel and whole-leaf materials. An MTT assay was performed to determine the cytotoxicity of the various aloe whole-leaf and gel materials on HaCaT cells. Wound healing and in vitro cell migration were investigated with HaCaT cells by means of the CytoSelect™ assay kit.
Results: The in vitro wound healing assay suggested that all the aloe gel and whole-leaf materials examined, exhibited faster wound healing activity than the untreated control group. After 48 h, all the aloe gel and whole-leaf materials almost completely caused full wound closure, displaying 98.07% (A. marlothii whole-leaf), 98.00% (A. vera gel), 97.20% (A. marlothii gel), 96.00% (A. vera whole-leaf), 94.00% (A. ferox gel) and 81.30% (A. ferox whole-leaf) wound closure, respectively. It was noteworthy that the gel materials of all the three aloe species exhibited significantly faster (p < 0.05) wound healing actions when compared to their respective whole-leaf materials at 32 h.
Conclusion: The gel and whole-leaf materials of A. vera, A. ferox and A. marlothii have shown the ability to heal wounds at a faster rate and to a larger extent than untreated keratinocytes. The MTT assay results suggested that the gel and whole-leaf materials of all the selected Aloe species showed negligible toxicity towards the HaCaT cells.

Objectives: The aim of this study was to investigate the in vitro permeation enhancement effects of the gel and whole leaf materials of Aloe vera, Aloe marlothii and Aloe ferox using ketoprofen as a marker compound. Methods: The... more

Objectives: The aim of this study was to investigate the in vitro permeation enhancement effects of the gel and whole leaf materials of Aloe vera, Aloe marlothii and Aloe ferox using ketoprofen as a marker compound. Methods: The permeation studies were conducted across excised female abdominal skin in Franz diffusion cells and the delivery of ketoprofen into the stratum corneum-epidermis and epidermis-dermis layers of the skin was investigated using a tape stripping technique. Key findings: Aloe vera gel showed the highest permeation enhancing effect on ketoprofen (enhancement ratio or ER = 2.551) when compared to the control group, followed by Aloe marlothii gel (ER = 1.590) and Aloe ferox whole leaf material (ER = 1.520). Non-linear curve fitting calculations indicated that the drug permeation enhancing effect of Aloe vera gel can be attributed to an increased partitioning of the drug into the skin, while Aloe ferox whole leaf modified the diffusion characteristics of the skin for ketoprofen. The tape stripping results indicated that Aloe marlothii whole leaf delivered the highest concentration of the ketoprofen into the different skin layers. Conclusions: Of the selected aloe species investigated, Aloe vera gel material showed the highest potential as transdermal drug penetration enhancer across human skin.