Animal Management Research Papers - Academia.edu (original) (raw)

2025, PLoS ONE

In several taxonomic groups, females mate with several males during a single reproductive cycle. Although there is evidence that polyandry provides some benefits to females, it often involves mortality costs. However, empirical evidences of mortality costs of polyandry have so far been reported only in invertebrates. Whether polyandry has mortality costs in vertebrates is currently unknown. In the present study, we aimed to fill the gap by investigating the relationships between the level of polyandry (measured either by male relative testes mass or the percentage of multiple paternities) and female patterns of mortality across mammals. While we found that the two metrics of female mortality co-varied with pace of life, we did not find any evidence that polyandry leads to either decreased median lifespan or increased aging rate in mammals. We discuss such an absence of detectable mortality costs of polyandry in female mammals in light of recent advances in the study of mammalian reproductive biology and life-history tactics.

2025, Animals

Companion animals are at risk of psychological (and physical) harm if outdated, incorrect or aversive methods of training or equipment are used during training and behavioural modification. Companion animal guardians often engage professional animal behaviour and training services, yet this industry is not regulated in New Zealand. A voluntary national accreditation and registration programme could act to protect the welfare of animals by robustly evaluating the experience, qualifications and training methods of industry professionals. To investigate industry readiness for a national accreditation programme, we conducted an online survey and analysed the responses of 262 animal trainers, behavioural consultants, dog safety educators, veterinarians and veterinary nurses. A national accreditation programme, promoting the use of reward-based training and behavioural modification techniques, was generally supported, especially by individuals holding qualifications and membership of prof...

2025, PLOS ONE

Obesity is associated with a low-grade inflammation including moderately increased serum levels of the acute phase protein serum amyloid A (SAA). In obesity, SAA is mainly produced from adipose tissue and serum levels of SAA are associated with insulin resistance. SAA has been described as a chemoattractant for inflammatory cells and adipose tissue from obese individuals contains increased numbers of macrophages. However, whether adipose tissue-derived SAA can have a direct impact on macrophage infiltration in adipose tissue or the development of insulin resistance is unknown. The aim of this study was to investigate the effects of adipose tissue-derived SAA1 on the development of insulin resistance and obesityrelated inflammation. We have previously established a transgenic mouse model expressing human SAA1 in the adipose tissue. For this report, hSAA1 +/2 transgenic mice and wild type mice were fed with a high fat diet or normal chow. Effects of hSAA1 on glucose metabolism were assessed using an oral glucose tolerance test. Real-time PCR was used to measure the mRNA levels of macrophage markers and genes related to insulin sensitivity in adipose tissue. Cytokines during inflammation were analyzed using a Proinflammatory 7-plex Assay. We found similar insulin and glucose levels in hSAA1 mice and wt controls during an oral glucose tolerance test and no decrease in mRNA levels of genes related to insulin sensitivity in adipose tissue in neither male nor female hSAA1 animals. Furthermore, serum levels of proinflammatory cytokines and mRNA levels of macrophage markers in adipose tissue were not increased in hSAA1 mice. Hence, in this model we find no evidence that adipose tissue-derived hSAA1 influences the development of insulin resistance or obesityrelated inflammation.

2025, PLOS ONE

Female sexual behavior is controlled by central estrogenic action in the ventromedial nucleus of the hypothalamus (VMN). This region plays a pivotal role in facilitating sex-related behavior in response to estrogen stimulation via neural activation by several neurotransmitters, including histamine, which participates in this mechanism through its strong neural potentiating action. However, the mechanism through which estrogen signaling is linked to the histamine system in the VMN is unclear. This study was undertaken to investigate the relationship between estrogen and histamine receptor subtype H1 (H1R), which is a potent subtype among histamine receptors in the brain. We show localization of H1R exclusively in the ventrolateral subregion of the female VMN (vl VMN), and not in the dorsomedial subregion. In the vl VMN, abundantly expressed H1R were mostly colocalized with estrogen receptor a. Intriguingly, H1R mRNA levels in the vl VMN were significantly elevated in ovariectomized female rats treated with estrogen benzoate. These data suggest that estrogen can amplify histamine signaling by enhancing H1R expression in the vl VMN. This enhancement of histamine signaling might be functionally important for allowing neural excitation in response to estrogen stimulation of the neural circuit and may serve as an accelerator of female sexual arousal.

2025, Quaternary International

The transition from hunter-gathering to food-producing societies in the Mediterranean zone of north Africa was complex and variable, likely influenced by local ecological conditions as well as the socio-economic origins of the population. The adoption of domestic plants and animals was piecemeal, with hunting and gathering continuing as an important part of local subsistence strategies. Here, we investigate the timing and extent of the adoption of agricultural practices, namely herding and cultivation, in three diverse coastal and inland Early Neolithic sites in the Mediterranean Maghreb region, namely Ifri Oudadane, Ifri n'Etsedda and Hassi Ouenzga. Results from absorbed lipid residues extracted from 306 potsherds from these sites are correlated with information from faunal and archaeobotanical assemblages. Our findings suggest that agricultural practices, regarded as being of Neolithic origin, were never fully adopted in the Maghreb but rather that these farmer/foragers adopted a range of strategies including low-level food production (exploiting cereals and animal products, including meat and milk), gathering of wild plants and marine shellfish, and hunting both small and large sized game. These broad-spectrum farmer/foragers were clearly both flexible and resourceful and likely adapted their subsistence practices to maximise resource availability in an increasingly unpredictable environment.

2025, PLoS ONE

Fatty acid composition is an important phenotypic trait in pigs as it affects nutritional, technical and sensory quality of pork. Here, we reported a genome-wide association study (GWAS) for fatty acid composition in the longissimus muscle and abdominal fat tissues of 591 White Duroc6Erhualian F 2 animals and in muscle samples of 282 Chinese Sutai pigs. A total of 46 loci surpassing the suggestive significance level were identified on 15 pig chromosomes (SSC) for 12 fatty acids, revealing the complex genetic architecture of fatty acid composition in pigs. Of the 46 loci, 15 on SSC5, 7, 14 and 16 reached the genome-wide significance level. The two most significant SNPs were ss131535508 (P = 2.48610 225 ) at 41.39 Mb on SSC16 for C20:0 in abdominal fat and ss478935891 (P = 3.29610 213 ) at 121.31 Mb on SSC14 for muscle C18:0. A metaanalysis of GWAS identified 4 novel loci and enhanced the association strength at 6 loci compared to those evidenced in a single population, suggesting the presence of common underlying variants. The longissimus muscle and abdominal fat showed consistent association profiles at most of the identified loci and distinct association signals at several loci. All loci have specific effects on fatty acid composition, except for two loci on SSC4 and SSC7 affecting multiple fatness traits. Several promising candidate genes were found in the neighboring regions of the lead SNPs at the genome-wide significant loci, such as SCD for C18:0 and C16:1 on SSC14 and ELOVL7 for C20:0 on SSC16. The findings provide insights into the molecular basis of fatty acid composition in pigs, and would benefit the final identification of the underlying mutations.

2025, PLoS ONE

Heat stress compromises production, fertility, and health of dairy cattle. One mitigation strategy is to select individuals that are genetically resistant to heat stress. Most of the negative effects of heat stress on animal performance are a consequence of either physiological adaptations to regulate body temperature or adverse consequences of failure to regulate body temperature. Thus, selection for regulation of body temperature during heat stress could increase thermotolerance. The objective was to perform a genome-wide association study (GWAS) for rectal temperature (RT) during heat stress in lactating Holstein cows and identify SNPs associated with genes that have large effects on RT. Records on afternoon RT where the temperature-humidity index was $78.2 were obtained from 4,447 cows sired by 220 bulls, resulting in 1,440 useable genotypes from the Illumina BovineSNP50 BeadChip with 39,759 SNP. For GWAS, 2, 3, 4, 5, and 10 adjacent SNP were averaged to identify consensus genomic regions associated with RT. The largest proportion of SNP variance (0.07 to 0.44%) was explained by markers flanking the region between 28,877,547 and 28,907,154 bp on Bos taurus autosome (BTA) 24. That region is flanked by U1 (28,822,883 to 28,823,043) and NCAD (28,992,666 to 29,241,119). In addition, the SNP at 58,500,249 bp on BTA 16 explained 0.08% and 0.11% of the SNP variance for 2-and 3-SNP analyses, respectively. That contig includes SNORA19, RFWD2 and SCARNA3. Other SNPs associated with RT were located on BTA 16 (close to CEP170 and PLD5), BTA 5 (near SLCO1C1 and PDE3A), BTA 4 (near KBTBD2 and LSM5), and BTA 26 (located in GOT1, a gene implicated in protection from cellular stress). In conclusion, there are QTL for RT in heat-stressed dairy cattle. These SNPs could prove useful in genetic selection and for identification of genes involved in physiological responses to heat stress.

2025, PLoS ONE

All organisms react to noxious and mechanical stimuli but we still lack a complete understanding of cellular and molecular mechanisms by which somatosensory information is transformed into appropriate motor outputs. The small number of neurons and excellent genetic tools make Drosophila larva an especially tractable model system in which to address this problem. We developed high throughput assays with which we can simultaneously expose more than 1,000 larvae per manhour to precisely timed noxious heat, vibration, air current, or optogenetic stimuli. Using this hardware in combination with custom software we characterized larval reactions to somatosensory stimuli in far greater detail than possible previously. Each stimulus evoked a distinctive escape strategy that consisted of multiple actions. The escape strategy was contextdependent. Using our system we confirmed that the nociceptive class IV multidendritic neurons were involved in the reactions to noxious heat. Chordotonal (ch) neurons were necessary for normal modulation of head casting, crawling and hunching, in response to mechanical stimuli. Consistent with this we observed increases in calcium transients in response to vibration in ch neurons. Optogenetic activation of ch neurons was sufficient to evoke head casting and crawling. These studies significantly increase our understanding of the functional roles of larval ch neurons. More generally, our system and the detailed description of wild type reactions to somatosensory stimuli provide a basis for systematic identification of neurons and genes underlying these behaviors.

2025, Quaternary International

2025, General Anthropology

According to Russian law, animals are objects of property. Pets and farm animals belong to their private owners, zoo animals to organizations, wild animals to the state. But street dogs belong to no one. Adopted in December 2018, Federal Law 498 "Concerning the Responsible Treatment of Animals" created a new juridical category: the "animal without an owner." As written, the law stipulates that such ownerless animals may not be euthanized or otherwise killed (although a recent amendment complicates matters), but only caught, castrated, vaccinated once against rabies, and then kept kenneled at the expense of the regional budgets, or else "returned to their former habitat." That is, to the streets. So, this midsized pack-hunting predator -Canis lupus familiaris -roams as it wishes. At least in some places: on the outskirts, in the working-class suburbs, in the cities' industrial zones.

2024, Quaternary International

The transition from hunter-gathering to food-producing societies in the Mediterranean zone of north Africa was complex and variable, likely influenced by local ecological conditions as well as the socioeconomic origins of the population. The adoption of domestic plants and animals was piecemeal, with hunting and gathering continuing as an important part of local subsistence strategies. Here, we investigate the timing and extent of the adoption of agricultural practices, namely herding and cultivation, in three diverse coastal and inland Early Neolithic sites in the Mediterranean Maghreb region, namely Ifri Oudadane, Ifri n'Etsedda and Hassi Ouenzga. Results from absorbed lipid residues extracted from 306 potsherds from these sites are correlated with information from faunal and archaeobotanical assemblages. Our findings suggest that agricultural practices, regarded as being of Neolithic origin, were never fully adopted in the Maghreb but rather that these farmer/foragers adopted a range of strategies including low-level food production (exploiting cereals and animal products, including meat and milk), gathering of wild plants and marine shellfish, and hunting both small and large sized game. These broad-spectrum farmer/foragers were clearly both flexible and resourceful and likely adapted their subsistence practices to maximise resource availability in an increasingly unpredictable environment.

2024, Quaternary International

The transition from hunter-gathering to food-producing societies in the Mediterranean zone of north Africa was complex and variable, likely influenced by local ecological conditions as well as the socioeconomic origins of the population. The adoption of domestic plants and animals was piecemeal, with hunting and gathering continuing as an important part of local subsistence strategies. Here, we investigate the timing and extent of the adoption of agricultural practices, namely herding and cultivation, in three diverse coastal and inland Early Neolithic sites in the Mediterranean Maghreb region, namely Ifri Oudadane, Ifri n'Etsedda and Hassi Ouenzga. Results from absorbed lipid residues extracted from 306 potsherds from these sites are correlated with information from faunal and archaeobotanical assemblages. Our findings suggest that agricultural practices, regarded as being of Neolithic origin, were never fully adopted in the Maghreb but rather that these farmer/foragers adopted a range of strategies including low-level food production (exploiting cereals and animal products, including meat and milk), gathering of wild plants and marine shellfish, and hunting both small and large sized game. These broad-spectrum farmer/foragers were clearly both flexible and resourceful and likely adapted their subsistence practices to maximise resource availability in an increasingly unpredictable environment.

2024, PLoS ONE

2024

This study aimed to identify the main obstacles and solutions for the implementation of humane slaughter standards for cattle in Brazil, analyzing the challenges and proposing strategies to improve animal welfare in livestock production. A literature review was used to summarize the existing scientific and regulatory literature on the subject that addresses different aspects of the Brazilian context. The main challenges identified were the lack of adequate infrastructure and equipment, the lack of training programs, cultural resistance to humane practices, insufficient financial incentives and limited monitoring. The lack of appropriate infrastructure and lack of training were identified as direct causes of animal suffering and low quality of the slaughter process, while economic and cultural barriers hinder the acceptance and adoption of the standards. The analysis suggests that the full implementation of these standards requires a systemic approach that integrates financial incentives, awareness campaigns, robust monitoring and government support for training and infrastructure. In conclusion, the study highlights that an effective transition to humane practices depends on coordinated actions that address challenges in an interdependent manner, providing an environment where standards can be applied in a sustainable and ethical manner. The recommendations provide support for the formulation of public policies that promote animal welfare and meet market demands, positioning the Brazilian livestock sector in a competitive and responsible manner. I.

2024, PLoS ONE

Benedenia infections caused by the monogenean fluke ectoparasite Benedenia seriolae seriously impact marine finfish aquaculture. Genetic variation has been inferred to play a significant role in determining the susceptibility to this parasitic disease. To evaluate the genetic basis of Benedenia disease resistance in yellowtail (Seriola quinqueradiata), a genome-wide and chromosome-wide linkage analyses were initiated using F 1 yellowtail families (n = 90 per family) based on a highdensity linkage map with 860 microsatellite and 142 single nucleotide polymorphism (SNP) markers. Two major quantitative trait loci (QTL) regions on linkage groups Squ2 (BDR-1) and Squ20 (BDR-2) were identified. These QTL regions explained 32.9-35.5% of the phenotypic variance. On the other hand, we investigated the relationship between QTL for susceptibility to B. seriolae and QTL for fish body size. The QTL related to growth was found on another linkage group (Squ7). As a result, this is the first genetic evidence that contributes to detailing phenotypic resistance to Benedenia disease, and the results will help resolve the mechanism of resistance to this important parasitic infection of yellowtail.

2024

This paper presents the results of stable carbon (δ 13 C) and nitrogen (δ 15 N) isotopic analysis of bulk bone collagen from faunal remains from the Bronze Age site of Kaymakçı in western Anatolia. We use the isotopic values in conjunction with zooarchaeological data and contextual archaeological information to examine human-environment interactions; in particular, animal management and husbandry. Middle and Late Bronze Age agricultural systems in western and central Anatolia remain poorly understood, and this research aims to contribute to rectifying this research gap. We obtained stable isotopic values from both wild and domestic species, including deer, hare, birds, catfish, dogs, pigs, caprids and cattle. The δ 13 C values range from −22.0‰ to −16.9‰ and the δ 15 N values range from 2.3‰ to 10.3‰. For cattle, the δ 13 C values suggest that some specimens were subject to different management strategies, likely related to feeding or herding patterns or even the movement of animals in and around the region through some exchange mechanism. There appears to be no fixed location or strategy for the management of caprids and, furthermore, there is evidence to suggest that the herding/management areas of some caprids overlapped with those of wild animals such as deer and hare. Variation in δ 15 N values for pigs may indicate that whilst some animals in the sampled assemblage were free-roaming, others were penned. For the pigs and some other herbivorous domesticated species variation in δ 15 N values may also point to the exploitation of the wetland areas around Lake Marmara.

2024

The present study conducted on "Studies on Management Practices Adopted by Gaushalas in Parbhani and Latur districts". Data were collected from 40 selected Gaushalas and grouped into 3 categories based on total number of animals as small (100 animals), medium (300 animals) and large (>300 animals), 15 Gaushalas in small whereas 15 Gaushalas medium and 10 Gaushalas in large category in Parbhani and Latur districts. About 37.50 percent of the Gaushalas possessed medium herd size (between 300 cattle), followed by 37.50 percent with small herd size (below 100 cattle) and 25.00 percent with large herd size (above 300 cattle). Gaushalas have also been identified as the centres for conservation of declining cattle breeds. It is important to mention that, majority (100%) of the Gaushalas comparised of indigenous cattle in general and among them most of them were found to be unproductive and old which could be related to their primary objective to serve the old, infirm and unproductive cattle. Among the indigenous cattle maintained in the Gaushalas, most of them were old and unproductive cattle in small (33.33%), medium (56.12%) and large sized Gaushalas (49.51%). For proper management of Gaushala and care of cattle, enough manpower is essential. The cattle's current feeding habits in the Gaushalas region of the research. It was noted that cattle in small, medium, and large Gaushalas were fed an average of 2-5 kg/day of dry fodder, 2.0-4.0 kg/day of green fodder, 0.1-0.4 kg/day of concentrate, and 50 gms of mineral mixture.

2024

Sistem perkandangan merupakan faktor yang sangat penting dalam pemeliharaan ayam parent stock. Kandang merupakan tempat tinggal ayam bibit untuk melakukan semua aktivitas selama hidupnya seperti makan, minum, tidur. Kandang berperan dalam memberikan kenyamanan kepada ayam agar menghasilkan ayam yang sehat dan berkualitas. Tujuan penulisan tugas akhir ini untuk mengetahui sistem perkandangan ayam parent stock di PT Super Unggas Jaya (Farm Lampung). Metode pelaksanaan kegiatan Praktik Kerja Lapangan (PKL) ini adalah mengikuti seluruh aktifitas yang ada diperusahaan tersebut dengan jadwal dan kegiatan yang telah ditetapkan, serta mengumpulkan data primer dan data sekunder. Berdasarkan hasil dan pembahasan yang telah dilakukan dapat disimpulkan bahwa untuk menghasilkan bibit ayam yang berkualitas, dibutuhkan manajemen perkandangan yang baik.

2024

The park elephants of different tiger reserves including Kanha, Panna and Bandhavgarh were incorporated for the assessment of cortisol levels in male, female and sub-adults groups. Overall cortisol level obtained 234.2 ng/g, 250.8 ng/g, 232.2 ng/g in Kanha tiger reserve, Panna tiger reserve and Bandhavgarh tiger reserve respectively. Out of 30 elephants, male and female shows no difference in level of cortisol however age wise assessment of cortisol level shows calves have least stress rather than adults and sub-adults.

2024

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2024, Dairy

This study aimed to identify suitable predictors of nitrogen (N) use efficiency (NUE; milk N/N intake) for cows that differed in breeds and were fed with ryegrass pasture, using existing data from the scientific literature. Data from 16 studies were used to develop models based on the relationships between NUE and dietary and animal-based factors. Data from a further 10 studies were used for model validation. Milk urea N (MUN) and dietary water-soluble carbohydrate-to-crudeprotein ratio (WSC/CP) were the best and most practical animal- and diet-based proxies to predict NUE. The results indicate that it might be necessary to adopt separate models for different breeds when using WSC/CP to predict NUE but not when using MUN.

2024, PLoS ONE

2024, PLOS ONE

Treatment with triglyceride emulsions of docosahexaenoic acid (tri-DHA) protected neonatal mice against hypoxia-ischemia (HI) brain injury. The mechanism of this neuroprotection remains unclear. We hypothesized that administration of tri-DHA enriches HI-brains with DHA/DHA metabolites. This reduces Ca 2+ -induced mitochondrial membrane permeabilization and attenuates brain injury. Methods 10-day-old C57BL/6J mice following HI-brain injury received tri-DHA, tri-EPA or vehicle. At 4-5 hours of reperfusion, mitochondrial fatty acid composition and Ca 2+ buffering capacity were analyzed. At 24 hours and at 8-9 weeks of recovery, oxidative injury, neurofunctional and neuropathological outcomes were evaluated. In vitro, hyperoxia-induced mitochondrial generation of reactive oxygen species (ROS) and Ca 2+ buffering capacity were measured in the presence or absence of DHA or EPA. Only post-treatment with tri-DHA reduced oxidative damage and improved short-and longterm neurological outcomes. This was associated with increased content of DHA in brain mitochondria and DHA-derived bioactive metabolites in cerebral tissue. After tri-DHA administration HI mitochondria were resistant to Ca 2+ -induced membrane permeabilization. In vitro, hyperoxia increased mitochondrial ROS production and reduced Ca 2+ buffering capacity; DHA, but not EPA, significantly attenuated these effects of hyperoxia.

2024

Free-roaming cats have a devastating impact on wildlife populations with stray/feral cats being the most problematic. In some areas, community members provide these cats with food, water, and shelter often in conjunction with a trap, neuter, return (TNR) program. Regardless of TNR, some studies suggest that feeding stray cats allows them to live longer which increases their impact on local wildlife populations. In contrast, we documented that a constantly available food source correlated to a higher density of cats in urban areas with less wildlife value and where they can be integrated into a TNR program. The Hayden Island Cat Project has utilized TNR, cat adoption, and outreach to humanely reduce the free-roaming cat population since 2014. Many residents on the island provide feeding stations for the cats and actively participate in the TNR program. To determine how feeding might affect cat spatial distribution, camera traps were placed at 20 different stations in urban and natura...

2024, The Rangeland Journal

2024, Studia Orientalia Electronica

Much of the literature on pastoralists and empire concerns mobile tribes and often focuses on imperial schemes of resettlement, or tribal thwarting of state initiatives. This submission argues that in mid-first-millennium bce Babylonia, large bureaucratic temples stood between the imperial state and Babylonia's mobile class of shepherds. This article then explores this dynamic further, focusing on the use of administrative information as a point of imperial contestation, examining issues of local control and clashing hierarchies as the shepherds served an imperial obligation in the Mesopotamian hinterland, and finally argues that the pastoral dynamic presented here is of a piece with the larger political role of the temple in Babylonian life-both urban, familiar, and central and at the same time distant, other-like, and enigmatic.

2024, Lai&al-2024-Intensified-animal-exploitation-Sardinia-CopperAge

In this paper, dietary patterns are reconstructed across two phases represented at the Copper Age of Scaba 'e Arriu (Sardinia, Italy) via isotopic analysis of human and animal skeletal remains. Collagen carbon, nitrogen, and bioapatite carbon, and oxygen isotopes were used to infer diet, economic practices, and possibly different climatic conditions. Differential management of cattle, sheep/goats, and pigs was detected, with scarce animal products in the human diet in the Early Copper Age, followed by an increase in animal products identifiable in herbivore livestock, coincident with more rainy conditions in the Late Copper Age. Dietary information was then considered in light of the climatic periods already known for the period and compatible with the data presented. The study's outcome indicates generally sedentary and endogamous groups with only a few outliers in both phases.

2024, PLoS ONE

Genetic diversity was assessed in samples of cultured Atlantic salmon, Salmo salar L., obtained from facilities in Chile between 2005 and 2010, a period of time during which the infectious pathogens Infectious Salmon Anemia (ISA) virus, Caligus rogercresseyi (sea lice), and Piscirickettsia salmonis (salmon rickettsial syndrome) were common. Two panels of microsatellite markers were utilized: one with microsatellites with no known gene associations (neutral) and one featuring microsatellites linked to putative immune-related genes (immune-related). Allelic richness and gene diversity across samples were significantly greater in neutral loci as compared to immune-related loci. Both diversity measures were homogeneous among samples for immune-related loci and heterogeneous among samples for neutral loci. Immune-related loci were identified as F ST outliers in pairwise comparisons of samples at a 10-fold higher frequency than neutral loci. These results indicate that neutral and immune-related portions of the Atlantic salmon genome may have differed in response to the gauntlet of pathogens and that monitoring of specific, well characterized immune-related loci as well as neutral loci in cultured species could be useful when disease control and prevention is a goal.

2024, PLoS Genetics

The domestication and development of cattle has considerably impacted human societies, but the histories of cattle breeds and populations have been poorly understood especially for African, Asian, and American breeds. Using genotypes from 43,043 autosomal single nucleotide polymorphism markers scored in 1,543 animals, we evaluate the population structure of 134 domesticated bovid breeds. Regardless of the analytical method or sample subset, the three major groups of Asian indicine, Eurasian taurine, and African taurine were consistently observed. Patterns of geographic dispersal resulting from co-migration with humans and exportation are recognizable in phylogenetic networks. All analytical methods reveal patterns of hybridization which occurred after divergence. Using 19 breeds, we map the cline of indicine introgression into Africa. We infer that African taurine possess a large portion of wild African auroch ancestry, causing their divergence from Eurasian taurine. We detect exportation patterns in Asia and identify a cline of Eurasian taurine/indicine hybridization in Asia. We also identify the influence of species other than Bos taurus taurus and B. t. indicus in the formation of Asian breeds. We detect the pronounced influence of Shorthorn cattle in the formation of European breeds. Iberian and Italian cattle possess introgression from African taurine. American Criollo cattle originate from Iberia, and not directly from Africa with African ancestry inherited via Iberian ancestors. Indicine introgression into American cattle occurred in the Americas, and not Europe. We argue that cattle migration, movement and trading followed by admixture have been important forces in shaping modern bovine genomic variation.

2024, PLOS ONE

Recently, the widespread distribution of pesticides detected in the hive has raised serious concerns about pesticide exposure on honey bee (Apis mellifera L.) health. A larval rearing method was adapted to assess the chronic oral toxicity to honey bee larvae of the four most common pesticides detected in pollen and wax -fluvalinate, coumaphos, chlorothalonil, and chloropyrifos -tested alone and in all combinations. All pesticides at hive-residue levels triggered a significant increase in larval mortality compared to untreated larvae by over two fold, with a strong increase after 3 days of exposure. Among these four pesticides, honey bee larvae were most sensitive to chlorothalonil compared to adults. Synergistic toxicity was observed in the binary mixture of chlorothalonil with fluvalinate at the concentrations of 34 mg/L and 3 mg/L, respectively; whereas, when diluted by 10 fold, the interaction switched to antagonism. Chlorothalonil at 34 mg/L was also found to synergize the miticide coumaphos at 8 mg/L. The addition of coumaphos significantly reduced the toxicity of the fluvalinate and chlorothalonil mixture, the only significant non-additive effect in all tested ternary mixtures. We also tested the common 'inert' ingredient N-methyl-2-pyrrolidone at seven concentrations, and documented its high toxicity to larval bees. We have shown that chronic dietary exposure to a fungicide, pesticide mixtures, and a formulation solvent have the potential to impact honey bee populations, and warrants further investigation. We suggest that pesticide mixtures in pollen be evaluated by adding their toxicities together, until complete data on interactions can be accumulated.

2024, Archaeological Research in Asia

The way that people manage their livestock tells us about their interactions with the landscape, particularly the nature of adaptation to specific environments, social organisation, resilience and long-term farming sustainability. Globally, there is considerable variation in how these practices are manifested, due to differences in water availability, levels of environmental diversity and aridity, and also the nature of cultural choices. South Asia's Indus Civilisation (c.3000-1500 BCE) provides an important opportunity for investigating how populations managed their animals, because the region shows considerable diversity in rainfall distribution, seasonality and intensity, which results in marked environmental variability that is susceptible to change over time. The latter is particularly significant when it comes to consideration of the impact of the 4.2 ka BP event and its relation to the deurbanisation of the Indus Civilisation. This paper presents carbon isotope data from animal teeth from nine archaeological sites distributed across northwest India that are suitable for exploring how diverse practices were, and how animal management strategies changed through time. These data show clear differentiation in feeding practices between species, with cattle and water buffalo consuming very high proportions of C 4 plants, while sheep and goat ate varying quantities of C 3 and C 4 plants. This pattern is generally consistent across sites and throughout different periods, suggesting that the strategy was adapted to a range of environmental conditions and settlements of different sizes. We suggest that humans controlled cow and water buffalo diets, and they were likely provided with fodder. In contrast, sheep and goats had a less controlled diet, and were presumably more likely to roam the landscape. These animal management strategies must have involved some separation of tasks, although it remains unclear if this was on a household, settlement or population level.

2024, Quaternary International

The transition from hunter-gathering to food-producing societies in the Mediterranean zone of north Africa was complex and variable, likely influenced by local ecological conditions as well as the socioeconomic origins of the population. The adoption of domestic plants and animals was piecemeal, with hunting and gathering continuing as an important part of local subsistence strategies. Here, we investigate the timing and extent of the adoption of agricultural practices, namely herding and cultivation, in three diverse coastal and inland Early Neolithic sites in the Mediterranean Maghreb region, namely Ifri Oudadane, Ifri n'Etsedda and Hassi Ouenzga. Results from absorbed lipid residues extracted from 306 potsherds from these sites are correlated with information from faunal and archaeobotanical assemblages. Our findings suggest that agricultural practices, regarded as being of Neolithic origin, were never fully adopted in the Maghreb but rather that these farmer/foragers adopted a range of strategies including low-level food production (exploiting cereals and animal products, including meat and milk), gathering of wild plants and marine shellfish, and hunting both small and large sized game. These broad-spectrum farmer/foragers were clearly both flexible and resourceful and likely adapted their subsistence practices to maximise resource availability in an increasingly unpredictable environment.

2024, PLOS ONE

Absorption of small water-soluble nutrients in vertebrate intestines occurs both by specific, mediated transport and by nonspecific, passive, paracellular transport. Although it is apparent that paracellular absorption represents a significant route for nutrient absorption in many birds and mammals, especially small, flying species, its importance in ectothermic vertebrates has not previously been explored. Therefore, we measured fractional absorption (e) and absorption rate of three paracellular probes (arabinose, L-rhamnose, cellobiose) and of 3-O-methyl D-glucose (absorbed by both mediated and paracellular pathways) by the large herbivorous lizard, Uromastyx aegyptia, to explore the relative importance of paracellular and mediated transport in an ectothermic, terrestrial vertebrate. Fractional absorption of 3-O-methyl D-glucose was high (e = 0.7360.04) and similar to other vertebrates; e of the paracellular probes was relatively low (arabinose e = 0.3160.03, Lrhamnose e = 0.1960.02, and cellobiose e = 0.1460.02), and decreased with molecular mass, a pattern consistent with other vertebrates. Paracellular absorption accounted for approximately 24% of total 3-O-methyl D-glucose uptake, indicating low reliance on this pathway for these herbivorous lizards, a pattern similar to that found in other terrestrial vertebrates, and different from small flying endotherms (both birds and bats).

2024, Journal of Archaeological Science: Reports

In this paper, we report the stable carbon and nitrogen isotope analysis results of wild (n = 15) and domestic (n = 21) animal samples from the Proezzhaia I site, a fortified Medieval settlement in Trans-Baikal, Siberia. Additionally, we analyzed five modern freshwater fish samples from the Shilka River, which flows immediately north of the site. Together, these samples provide the first dietary stable isotope data for the entire Trans-Baikal region. Our results reveal a C 3 plant dominated diet for all animal taxa sampled, but with a possible minor inclusion of C 4 plant material in the diets of some cattle (Bos sp.) and horses (Equus sp.). We infer this C 4 plant was likely a type of millet, the remains of which were identified at a contemporaneous site upriver. Additionally, these results indicate that some cattle and horses consumed plant material grown in 15 N-enriched soil, which suggests several distinct animal management practices, or may indicate a trade in or movement of animals from an area with a different nitrogen isotope baseline. 1.1. Archaeological context Proezzhaia I is located on a terrace along the southeastern bank of

2024, Animals

The compost-bedded pack barn (CBPB) system has been increasingly adopted by dairy farms due to its ability to enhance animal comfort and milk production. This study evaluated the associations among bedding characteristics, milk quality and composition, and subclinical mastitis (SCM) occurrence in dairy herds housed in CBPB systems. Over a period of six months, data related to milk quality and udder health and bedding sampling were collected from eight dairy farms. Monthly measurements of the bedding temperature and wind speed inside the CBPB were taken, while temperature and relative humidity data inside the CBPB were recorded using a datalogger. Bedding samples were subjected to analysis of moisture, pH, microbiological count, and carbon/nitrogen ratio. Data on milk composition (fat, protein, milk urea nitrogen, and total solids) and quality (somatic cell count and standard plate count) of bulk tank milk were obtained from DHIA results. Canonical correlation analyses were used to e...

2024, PLoS ONE

The body of most fishes is fully covered by scales that typically form tight, partially overlapping rows. While some of the genes controlling the formation and growth of fish scales have been studied, very little is known about the genetic mechanisms regulating scale pattern formation. Although the existence of two genes with two pairs of alleles (S&s and N&n) regulating scale coverage in cyprinids has been predicted by Kirpichnikov and colleagues nearly eighty years ago, their identity was unknown until recently. In 2009, the 'S' gene was found to be a paralog of fibroblast growth factor receptor 1, fgfr1a1, while the second gene called 'N' has not yet been identified. We re-visited the original model of Kirpichnikov that proposed four major scale pattern types and observed a high degree of variation within the so-called scattered phenotype due to which this group was divided into two sub-types: classical mirror and irregular. We also analyzed the survival rates of offspring groups and found a distinct difference between Asian and European crosses. Whereas nude 6 nude crosses involving at least one parent of Asian origin or hybrid with Asian parent(s) showed the 25% early lethality predicted by Kirpichnikov (due to the lethality of the NN genotype), those with two Hungarian nude parents did not. We further extended Kirpichnikov's work by correlating changes in phenotype (scale-pattern) to the deformations of fins and losses of pharyngeal teeth. We observed phenotypic changes which were not restricted to nudes, as described by Kirpichnikov, but were also present in mirrors (and presumably in linears as well; not analyzed in detail here). We propose that the gradation of phenotypes observed within the scattered group is caused by a gradually decreasing level of signaling (a dosedependent effect) probably due to a concerted action of multiple pathways involved in scale formation.

2024, PLOS ONE

Biologists routinely use molecular markers to identify conservation units, to quantify genetic connectivity, to estimate population sizes, and to identify targets of selection. Many imperiled eagle populations require such efforts and would benefit from enhanced genomic resources. We sequenced, assembled, and annotated the first eagle genome using DNA from a male golden eagle (Aquila chrysaetos) captured in western North America. We constructed genomic libraries that were sequenced using Illumina technology and assembled the high-quality data to a depth of ,40x coverage. The genome assembly includes 2,552 scaffolds .10 Kb and 415 scaffolds .1.2 Mb. We annotated 16,571 genes that are involved in myriad biological processes, including such disparate traits as beak formation and color vision. We also identified repetitive regions spanning 92 Mb (,6% of the assembly), including LINES, SINES, LTR-RTs and DNA transposons. The mitochondrial genome encompasses 17,332 bp and is ,91% identical to the Mountain Hawk-Eagle (Nisaetus nipalensis). Finally, the data reveal that several anonymous microsatellites commonly used for population studies are embedded within protein-coding genes and thus may not have evolved in a neutral fashion. Because the genome sequence includes ,800,000 novel polymorphisms, markers can now be chosen based on their proximity to functional genes involved in migration, carnivory, and other biological processes.

2024, PLoS ONE

2024, PLOS ONE

Small-scale commercial poultry production is emerging as an important form of livestock production in Africa, providing sources of income and animal protein to many poor households, yet the occurrence and impact of coccidiosis on this relatively new production system remains unknown. The primary objective of this study was to examine Eimeria parasite occurrence on small-scale commercial poultry farms in Ghana, Tanzania and Zambia. Additionally, farm economic viability was measured by calculating the farm gross margin and enterprise budget. Using these economic measures as global assessments of farm productivity, encompassing the diversity present in regional husbandry systems with a measure of fundamental local relevance, we investigated the detection of specific Eimeria species as indicators of farm profitability. Faecal samples and data on production parameters were collected from small-scale (less than 2,000 birds per batch) intensive broiler and layer farms in peri-urban Ghana, Tanzania and Zambia. All seven Eimeria species recognised to infect the chicken were detected in each country. Furthermore, two of the three genetic variants (operational taxonomic units) identified previously in Australia have been described outside of Australia for the first time. Detection of the most pathogenic Eimeria species associated with decreased farm profitability and may be considered as an indicator of likely farm performance. While a causal link remains to be demonstrated, the presence of highly pathogenic enteric parasites may pose a threat to profitable, sustainable small-scale poultry enterprises in Africa.

2024, Archaeological Research in Asia

2024

This work aims the hoof morphological, physical, chemical, and mineralogical characteristics in Anglo Arabian horses, and Monterufoli ponies reared in Tuscany. 28 nail samples from wall and sole of hoof were analysed. All feet were healthy and well conformed. The hoof of Monterufoli Pony was more cylindrical, and the Anglo Arabian hoof was harder (H 112.8±4.9, and H 119.4±2.7 in sole and in wall respectively). The percentage of dry matter (83.03±0.67) was greater in Anglo-Arabian hoof, while the percentage in crude protein and in ashes in hoof was similar between breeds. The minerals were in greatest concentration in the Anglo Arabian hoof wall. Monterufoli Pony hoof seemed to excrete the minerals through the probable osmoregulation activity of Na, which has shown negative correlation with many minerals, and through the nail consumption, that is softer than that of Anglo Arabian. In principal component analyses of test results for both breeds, 10 significant factors (ratio of variance = 96.88) and 5 factors that explain 79.65% of cumulative variance were found. PCA analysis has shown a quite separation between breeds, confirming for the Anglo Arabian hoof the greater trend to accumulate minerals on nails, and for Monterufoli Pony hoof the antagonist activity of Sodium (Na) against other minerals.

2024, PLOS ONE

2024, PLoS ONE

2024, Animals

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

2024, Rangeland Journal

With the commercial development of the global positioning system (GPS), it is now possible to monitor the distribution of free ranging cattle and derive measures to describe landscape use. Animal GPS data can be integrated with a geographic information system (GIS) detailing topography, vegetation, soil type and other landscape features. Combining GPS and GIS information is useful for understanding how animals respond to spatial variability. This study quantified landtype preferences for Brahman cross steers over three time periods, from October 2004 to March 2006 in a replicated trial, under heavy (4 ha/AE; animal equivalent of~450 kg steer) and light (8 ha/AE) stocking in four,~105 ha paddocks of subtropical semi-arid savanna near Charters Towers, Queensland, Australia. The grazing trail was conducted at a scale much less than would be found in commercial situations. Consequently, the spatial pattern of cattle reported here may not represent what occurs at a commercial scale and implications are discussed. Results were analysed in terms of the spatial distribution of steers fitted with GPS devices in each of the four paddocks and for each stocking rate to provide insight into cattle distribution and land-type preferences. Steers walked in excess of 6 km per day, regardless of stocking rate, and exhibited diurnal patterns of movement, with peak activity around dawn (0500-0700 hours) and dusk (1800-2000 hours). The spatial distribution of the collared steers was not uniform and appeared to be strongly influenced by the prevailing drought conditions and location of water points within each paddock. A hierarchy of drivers for distribution was identified. With the exception of drinking water location, land subtype based on soil-vegetation associations influenced animal distribution. Preference indices (ŵ i) indicated that steers selected sites associated with heavy clay and texture contrast soils dominated by Eucalyptus coolabah Blakely & Jacobs (ŵ i = 5.33) and Eucalyptus brownii Maiden & Cambage (ŵ i = 3.27), respectively, and avoiding Eucalyptus melanophloia F.Muell. ridges (ŵ i = 0.26) and Eucalyptus cambageana Maiden (ŵ i = 0.12) on sodosols. The results suggest that spatial variation in cattle distribution within a paddock may be more critical than overall stocking rate in influencing the pattern of biomass utilisation. However, to quantifying the effects of different grazing land management practices on animal distribution on a commercial scale, additional studies in extensive paddocks are required.

2024, PLoS ONE

The functional trait-based approach is increasingly used to predict responses of ecological communities to disturbances, but most studies target a single taxonomic group. Here, we assessed the resilience of a forest ecosystem to an overabundant herbivore population by assessing changes in 19 functional traits for plant, 13 traits for ground beetle and 16 traits for songbird communities after six years of controlled browsing on Anticosti Island (Quebec, Canada). Our results indicated that plants were more responsive to 6 years of reduced browsing pressure than ground beetles and songbirds. However, co-inertia analysis revealed that ground beetle communities responded in a similar way than plant communities with stronger relationships between plant and ground beetle traits at reduced deer density, a pattern not detected between plant and songbird. High deer density favored plants species that reproduce vegetatively and with abiotic pollination and seed dispersal, traits implying little interaction with animal. On the other hand, traits found at reduced deer density mostly involved trophic interaction. For example, plants in this treatment had fleshy fruits and large seeds dispersed by birds or other animals whereas ground beetle species were carnivorous. Overall, our results suggest that plant communities recovered some functional components to overabundant herbivore populations, since most traits associated with undisturbed forests were reestablished after six years of deer reduction. The re-establishment of functional plant communities with traits involving trophic interaction induces changes in the ground-beetle trait community, but forest structure remains likely insufficiently heterogeneous to shift the songbird trait community within six years.

2024, Animals

In urban and peri-urban areas of the world, free-roaming cats often pose management challenges for authorities. Most are wandering owned or semi-owned cats (fed by people who do not perceive ownership). Some are lost or abandoned, or unowned cats who obtain food from humans unintentionally. Unidentified cats are classified as “stray” in shelter data, and by government agencies as “stray” or “feral” based on their behaviour. However, legally feral cats are usually considered to live and reproduce in the wild with no support from humans. Cats classified as feral in Australia can be managed using lethal methods, including shooting, poisoning, trapping, and blunt trauma. The impact of killing animals on shelter staff is well documented. However, no previous research has investigated psychological impacts of lethal cat management on citizens who care for free-roaming cats. Using semi-structured interviews, this study explored the lived experience of six cat caregivers affected by lethal ...

2024, Frontiers in Psychology

2024, South African Journal of Wildlife Research - 24-month delayed open access

The ability of cats (Felis catus) to colonize most land habitats worldwide led to an increasing number of feral cat populations in many areas where food resources are easily available. High densities of feral cats in urban areas, particularly in conservancies, have the potential to impact negatively on both human and local wildlife populations. Of particular interest was the Howard College Campus of the University of KwaZulu-Natal, a registered conservancy where there are differing opinions concerning the resident feral cat population. Consequently methods of controlling feral cat populations and the implications of these methods were reviewed. Despite various methods of feral cat population control existing there are two basic categories: either eradication or reproductive regulation. It is suggested that to control the feral cat population effectively in this urban conservancy, a suitable and ongoing sterilization programme, that is run in conjunction with a feral cat feeding programme, needs to be implemented. Both programmes need to be long-term and overseen by management. The feral cat population needs to be maintained at a level that allows the lowest migration rate into the conservancy, as well as a predation rate that will not negatively affect the resident wildlife populations. This may require some removal of feral cats at the start of a programme. Whatever management actions are followed, a monitoring programme must be put in place to document how effective the actions are.