DNA hybridization Research Papers - Academia.edu (original) (raw)

Endocarditis of native aortic and mitral valves due to an organism identified as Corynebacterium accolens developed in a 73-year-old patient without predisposing factors. The organism was identified asC. accolensby biochemical... more

Endocarditis of native aortic and mitral valves due to an organism identified as Corynebacterium accolens developed in a 73-year-old patient without predisposing factors. The organism was identified asC. accolensby biochemical identification, amplified rRNA gene restriction analysis, and DNA-DNA hybridization. This is the first case of C. accolens endocarditis reported, adding to the increasing number of Corynebacterium-related cases of endocarditis. Cases of endocarditis are rarely caused by corynebacteria. A total of 126 cases have been reviewed recently (4). Fifty-three ofthesecaseswererelatedtothepresenceofprostheticvalves, and 107 of the organisms were related to Corynebacterium species living commensally on the skin. Both associations may be ascribed to surgery or to the injection of drugs. Further- more,mostofthecasesoccurinpatientswithpreexistingheart disease or in patients who are parenteral drug users (4). The increase in the number of reports concerning Coryne- bacteriumi...

An integrated thermofluidic analysis of DNA hybridization, in the presence of combined electrokinetically and/or pressure-driven microchannel flows, is presented in this work. A comprehensive model is developed that combines bulk and... more

An integrated thermofluidic analysis of DNA hybridization, in the presence of combined electrokinetically and/or pressure-driven microchannel flows, is presented in this work. A comprehensive model is developed that combines bulk and surface transport of momentum, heat and solute with the pertinent hybridization kinetics, in a detailed manner. Results confirm that electrokinetic accumulation of DNA occurs within a few seconds or minutes, as compared to passive hybridization that could sometimes take several hours. Further, it is observed that by increasing the accumulation time, significantly higher concentration of DNA can be achieved at the capture probes. However, this eventually tends to attain a saturation state, due to a lesser probability of successful hybridization on account of a prior accumulation of target DNA molecules on the capture probe strands. While favorable pressure gradients augment DNA hybridization rates that are otherwise established by the electro-osmotic transport, adverse pressure gradients of comparable magnitude may turn out to be much less consequential in retarding the same. Such effects can be of potential significance in the designing of a microfluidic arrangement to achieve the fastest rate of DNA hybridization.

Strains traditionally identified as Proteus vulgaris formed three biogroups. Biogroup 1, characterized by negative reactions for indole production, salicin fermentation and aesculin hydrolysis, is now known as Proteus penneri. Biogroup 2,... more

Strains traditionally identified as Proteus vulgaris formed three biogroups. Biogroup 1, characterized by negative reactions for indole production, salicin fermentation and aesculin hydrolysis, is now known as Proteus penneri. Biogroup 2, characterized by positive reactions for indole, salicin and aesculin, was shown by DNA hybridization (hydroxyapatite method) to be a genetic species separate from biogroup 1 and from biogroup 3 which is positive for indole production and negative for salicin and aesculin. In this study, 52 strains were examined, of which 36 strains were Proteus vulgaris biogroup 3, which included the current type strain of the species P. vulgaris (ATCC 29905T), and compared to seven strains of Proteus vulgaris biogroup 2 and nine type strains of other species in the genera Proteus, Providencia and Morganella. By DNA hybridization, these 36 strains were separated into four distinct groups, designated as Proteus genomospecies 3, 4, 5 and 6. DNAs within each separate ...

Hybrid composites are advance conceptual composite materials. These are composite materials are the material which contains two or more different types of natural/synthetic fiber in which one type of fiber could neutralize with what are... more

Hybrid composites are advance conceptual composite materials. These are composite materials are the material which contains two or more different types of natural/synthetic fiber in which one type of fiber could neutralize with what are lacking in the other. Hybridization of natural fibers is stronger and high corrosion resistance than synthetic fibers like glass can improve the various mechanical and chemical useful properties. This paper makes an attempt to set a review on hybrid polymer composites reinforced with glass and bamboo fiber based on their biodegradability. The importance of biodegradability is to develop composite materials which are ecofriendly

While developing an effective vaccine can take months up to years, detection of infected patients seems like one of the best ideas for controlling the situation. The role of biosensors in containing highly pathogenic viruses, saving lives... more

While developing an effective vaccine can take months up to years, detection of infected patients seems like one of the best ideas for controlling the situation. The role of biosensors in containing highly pathogenic viruses, saving lives and economy is evident. A new competitive numerical platform specifically for designing microfluidic-integrated biosensors is developed and presented in this work. Properties of the biosensor, sample, buffer fluid and even the microfluidic channel can be modified in this model. This feature provides the scientific community with the ability to design a specific biosensor for requested point-of-care (POC) applications. First, the validation of the presented numerical platform against experimental data and then results and discussion, highlighting the important role of the design parameters on the performance of the biosensor is presented. For the latter, the baseline case has been set on the previous studies on the biosensors suitable for SARS-CoV, which has the highest similarity to the 2019 nCoV. Subsequently, the effects of concentration of the targeted molecules in the sample, installation position and properties of the biosensor on its performance were investigated in 11 case studies. The presented numerical framework provides an insight into understanding of the virus reaction in the design process of the biosensor and enhances our preparation for any future outbreaks. Furthermore, the integration of biosensors with different devices for accelerating the process of defeating the pandemic is proposed.

While developing an effective vaccine can take months up to years, detection of infected patients seems like one of the best ideas for controlling the situation. The role of biosensors in containing highly pathogenic viruses, saving lives... more

While developing an effective vaccine can take months up to years, detection of infected patients seems like one of the best ideas for controlling the situation. The role of biosensors in containing highly pathogenic viruses, saving lives and economy is evident. A new competitive numerical platform specifically for designing microfluidic-integrated biosensors is developed and presented in this work. Properties of the biosensor, sample, buffer fluid and even the microfluidic channel can be modified in this model. This feature provides the scientific community with the ability to design a specific biosensor for requested point-of-care (POC) applications. First, the validation of the presented numerical platform against experimental data and then results and discussion, highlighting the important role of the design parameters on the performance of the biosensor is presented. For the latter, the baseline case has been set on the previous studies on the biosensors suitable for SARS-CoV, ...

Numerical taxonomic, DNA-DNA hybridization, and phospholipid fatty acid composition analyses were performed on an extensive range of methanotrophic strains, including reference strains and environmental isolates obtained from sites... more

Numerical taxonomic, DNA-DNA hybridization, and phospholipid fatty acid composition analyses were performed on an extensive range of methanotrophic strains, including reference strains and environmental isolates obtained from sites throughout eastern Australia. When the results of these studies were related to the results of a study based on genomic physicochemical properties, they clarified group I and I1 methanotroph genus and species

The systematic position of the alkaliphilic and halotolerant strain Bacillus sp. FTU was refined in view of the comprehensive taxonomic revision of the group of alkaliphilic and alkalitolerant Bacillus strains. Sequence analysis of almost... more

The systematic position of the alkaliphilic and halotolerant strain Bacillus sp. FTU was refined in view of the comprehensive taxonomic revision of the group of alkaliphilic and alkalitolerant Bacillus strains. Sequence analysis of almost the entire 16S rRNA gene of Bacillus sp. FTU revealed 99.8% homology with two Bacillus pseudofirmus strains. Subsequent DNA–DNA hybridization analysis confirmed the close relationship of Bacillus sp. FTU with the type strain of B. pseudofirmus (the level of homology reached 86%). Results of physiological and biochemical characterizations relevant for the group clearly underlined the positioning of strain FTU within this species. It is therefore concluded that Bacillus sp. FTU represents a strain of the alkaliphilic species B. pseudofirmus and is to be renamed as B. pseudofirmus FTU. The phylogeny of different Bacillus species is discussed using N-terminal sequence homologies of some caa3-type oxidase subunits.

Bacterial strains isolated from the genital tracts of humans (predominantly males), semen of boars, and uterine and vaginal secretions of sows were identified as Corynebacterium glucuronolyticum and were compared with the type strains of... more

Bacterial strains isolated from the genital tracts of humans (predominantly males), semen of boars, and uterine and vaginal secretions of sows were identified as Corynebacterium glucuronolyticum and were compared with the type strains of the recently proposed species Corynebacterium glucuronolyticum and Corynebacterium seminale. The two type strains as well as the clinical strains were shown by DNA-DNA hybridization and sequencing of the 16S rRNA gene to be related at the species level. All strains were classified as C. glucuronolyticum, because this name has nomenclatural priority over C. seminale.

Magnetic beads (MBs) are versatile tools in the separation of nucleic acids, proteins and other biomacromolecules, their complexes and cells. In this article recent application of MBs in electrochemical biosensing and particularly in the... more

Magnetic beads (MBs) are versatile tools in the separation of nucleic acids, proteins and other biomacromolecules, their complexes and cells. In this article recent application of MBs in electrochemical biosensing and particularly in the development of DNA hybridization sensors is reviewed. In these sensors MBs serve not only for separation but also as a platform for optimized DNA hybridization. A