Structure Elucidation Research Papers - Academia.edu (original) (raw)

Six triterpenes, 3β-acetoxy-12,19-dioxo-13(18)-oleanene (1), 3β-acetoxy-19(29)-taraxasten-20α-ol (2), 3β-acetoxy-21α,22α-epoxytaraxastan-20α-ol (3), 3,22-dioxo-20-taraxastene (4), 3β-acetoxy-11α,12α-epoxy-16-oxo-14-taraxerene (5), 3β-acetoxy-25-methoxylanosta-8,23-diene (6) along with nine known triterpenes, 3β-acetoxy-11α,12α-epoxy-14-taraxerene (7), 3β-acetoxy-25-hydroxylanosta-8,23-diene (8), oleanonic acid (9), acetylbetulinic acid (10), betulonic acid (11), acetylursolic acid (12), ursonic acid (13), ursolic acid (14), and 3-oxofriedelan-28-oic acid (15) were isolated from the aerial roots of Ficus microcarpa, and their structures elucidated by spectroscopic methods. The in vitro cytotoxic efficacy of these triterpenes was investigated using three human cancer cell lines, namely, HONE-1 nasopharyngeal carcinoma, KB oral epidermoid carcinoma, and HT29 colorectal carcinoma cells. Compound 8 and pentacyclic triterpenes 9–15 possessing a carboxylic acid functionality at C-28 showed significant cytotoxic activities against the aforementioned cell lines and gave IC50 values in the range 4.0–9.4 μM.Six triterpenes along with nine known triterpenes were isolated from the aerial roots of Ficus microcarpa, and their structures elucidated by spectroscopic methods. The in vitro cytotoxic efficacy of these triterpenes against three human cancer cell lines was investigated.