Cellosaurus cell line Huh-7 clone 5-15 (CVCL_C8QS) (original) (raw)

Cell line name

Huh-7 clone 5-15

Accession

CVCL_C8QS

To cite this cell line use: Huh-7 clone 5-15 (RRID:CVCL_C8QS)

Comments

Population: Japanese.
Virology: Harbors a self-replicating bicistronic hepatitis C virus (HCV) genotype 1b subgenomic replicon (PubMed=10390360; PubMed=11152498).
Doubling time: 25.5 +- 2.5 hours (PubMed=11152498).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: In situ; Liver; UBERON=UBERON_0002107.

Sequence variations

Mutation; HGNC; 6307; KDR; Simple; p.Gln472His (c.1416A>T); ClinVar=VCV000134603; Zygosity=Homozygous (from parent cell line).
Mutation; HGNC; 20932; POLD3; Simple; p.Lys109Arg (c.326A>G) (p.Lys70Arg, c.209A>G); Zygosity=Unspecified (from parent cell line).
Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
Mutation; HGNC; 11998; TP53; Simple; p.Tyr220Cys (c.659A>G); ClinVar=VCV000127819; Zygosity=Homozygous (from parent cell line).

Disease

Adult hepatocellular carcinoma (NCIt: C7956)
Adult hepatocellular carcinoma (ORDO: Orphanet_210159)

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_0336 (Huh-7)

Sex of cell

Male

Age at sampling

57Y