Cellosaurus cell line HCT 116 CDKN1A(-/-) (CVCL_HD73) (original) (raw)

Cell line name

HCT 116 CDKN1A(-/-)

Synonyms

CDKN1A (-/-) HCT116; HCT116 p21-/-; 80S14

Accession

CVCL_HD73

Resource Identification Initiative

To cite this cell line use: HCT 116 CDKN1A(-/-) (RRID:CVCL_HD73)

Comments

Population: Caucasian.
Knockout cell: Method=Homologous recombination; HGNC; HGNC:1784; CDKN1A.
Caution: There are two clones of p21-/- cells that were created, 80S4 and 80S14. It is not clear which clone this cell line corresponds to, but as 80S14 was much more used than 80S4 we believe it is that clone.
Derived from site: In situ; Colon; UBERON=UBERON_0001155.

Sequence variations

Mutation; HGNC; HGNC:173; ACVR2A; Simple; p.Lys437Argfs*5 (c.1310delA); dbSNP=rs764719749; Zygosity=Homozygous (from parent cell line).
Mutation; HGNC; HGNC:1101; BRCA2; Simple; p.Ile2675Aspfs*6 (c.8021dupA) (c.8021_8022insA); ClinVar=VCV000267050; Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; HGNC:1787; CDKN2A; Simple; p.Arg24Serfs*20 (c.68dupG) (c.68_69insG) (p.G23fs); Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; HGNC:2514; CTNNB1; Simple; p.Ser45del (c.133_135delTCT); ClinVar=VCV000017576; Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; HGNC:3373; EP300; Simple; p.Met1470Cysfs*22 (c.4408delA); Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; HGNC:3373; EP300; Simple; p.Asn1700Thrfs*9 (c.5099delA); Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.His1047Arg (c.3140A>G); ClinVar=VCV000013652; Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; HGNC:9277; PPM1D; Simple; p.Leu450Ter (c.1349delT) (p.Leu450fs) (c.1344delT); Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; HGNC:11773; TGFBR2; Simple; p.Lys128Serfs*35 (c.383delA); ClinVar=VCV000477546; Zygosity=Homozygous (from parent cell line).

Disease

Colon carcinoma (NCIt: C4910)

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_0291 (HCT 116)

Sex of cell

Male

Age at sampling

48Y