Cellosaurus cell line HeLa GFP-LC3-RFP-LC3deltaG (CVCL_UP51) (original) (raw)

Cell line name

HeLa GFP-LC3-RFP-LC3deltaG

Synonyms

GFP-LC3-RFP-LC3deltaG HeLa

Accession

CVCL_UP51

Resource Identification Initiative

To cite this cell line use: HeLa GFP-LC3-RFP-LC3deltaG (RRID:CVCL_UP51)

Comments

Population: African American.
Characteristics: Stably expresses EGFP-LC3-mRFP-LC3delta, an autophagic flux probe. GFP-LC3 is fused to the N-terminus of RFP-LC3 whose C-terminal glycine is deleted. When expressed, the probe it is separated into EGFP-LC3 and mRFP-LC3deltaG by the action of endogenous ATG4 family proteases that cleave the peptide bond after the C-terminal glycine of LC3 in the cytosol producing equimolar amounts of EGFP-LC3 and mRFP-LC3deltaG.
Genetic integration: Method=Transfection/transduction; Gene=RGD; 735183; Map1lc3a.
Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transfection/transduction; Gene=FPbase; 5YCFA; mRFP1 (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.

Disease

Human papillomavirus-related cervical adenocarcinoma (NCIt: C27677)

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_0030 (HeLa)

Sex of cell

Female

Age at sampling

30Y6M