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		PDBsum entry 1frx Go to PDB code: Electron transport PDB id 1frx Loading ... Contents Protein chain 106 a.a.* Ligands SF4 F3S Waters ×22 * Residue conservation analysis PDB id: 1frx Links PDBe RCSB MMDB JenaLib Proteopedia CATH SCOP PDBSWS PDBePISA CSA ProSAT Name: Electron transport Title: Structure and properties of c20s fdi mutant Structure: Ferredoxin. Chain: a. Engineered: yes Source: Azotobacter vinelandii. Organism_taxid: 354. Resolution: 2.50Å R-factor: 0.229 Authors: C.D.Stout Key ref: B.Shen et al. (1995). Site-directed mutagenesis of Azotobacter vinelandii ferredoxin I: cysteine ligation of the [4Fe-4S] cluster with protein rearrangement is preferred over serine ligation.Proc Natl Acad Sci U S A,92, 10064-10068.PubMed id: 7479727 DOI: 10.1073/pnas.92.22.10064 Date: 13-Jul-94 Release date: 31-Aug-94 PROCHECK Headers References Protein chain ? P00214 (FER1_AZOVI) - Ferredoxin-1 from Azotobacter vinelandii Seq:Struc: 107 a.a. 106 a.a.* Key: PfamA domain Secondary structure CATH domain * PDB and UniProt seqs differ at 1 residue position (black cross) DOI no: 10.1073/pnas.92.22.10064 Proc Natl Acad Sci U S A 92:10064-10068 (1995) PubMed id: 7479727 Site-directed mutagenesis of Azotobacter vinelandii ferredoxin I: cysteine ligation of the [4Fe-4S] cluster with protein rearrangement is preferred over serine ligation. B.Shen, D.R.Jollie, T.C.Diller, C.D.Stout, P.J.Stephens, B.K.Burgess. ABSTRACT The [4Fe-4S] cluster of Azotobacter vinelandii ferredoxin I receives three of its four ligands from a Cys-Xaa-Xaa-Cys-Xaa-Xaa-Cys sequence at positions 39-45 while the fourth ligand, Cys20, is provided by a distal portion of the sequence. Previously we reported that the site-directed mutation of Cys20 to Ala (C20A protein) resulted in the formation of a new [4Fe-4S] cluster that obtained its fourth ligand from Cys24, a free cysteine in the native structure. That ligand exchange required significant protein rearrangement. Here we report the conversion of Cys20 to Ser (C20S protein), which gives the protein the opportunity either to retain the native structure and use the Ser20 O gamma as a ligand or to rearrange and use Cys24. X-ray crystallography demonstrates that the cluster does not use the Ser20 O gamma as a ligand; rather it rearranges to use Cys24. In the C20S protein the [4Fe-4S] cluster has altered stability and redox properties relative to either C20A or the native protein. Literature references that cite this PDB file's key reference PubMed id Reference 15181002 S.Agarwalla, R.M.Stroud, and B.J.Gaffney (2004). Redox reactions of the iron-sulfur cluster in a ribosomal RNA methyltransferase, RumA: optical and EPR studies. J Biol Chem,279, 34123-34129. 11170391 D.N.Heaton, G.N.George, G.Garrison, and D.R.Winge (2001). The mitochondrial copper metallochaperone Cox17 exists as an oligomeric, polycopper complex. Biochemistry,40, 743-751. 11054113 R.Bingemann, and A.Klein (2000). Conversion of the central [4Fe-4S] cluster into a [3Fe-4S] cluster leads to reduced hydrogen-uptake activity of the F420-reducing hydrogenase of Methanococcus voltae. Eur J Biochem,267, 6612-6618. 10387068 C.G.Schipke, D.B.Goodin, D.E.McRee, and C.D.Stout (1999). Oxidized and reduced Azotobacter vinelandii ferredoxin I at 1.4 A resolution: conformational change of surface residues without significant change in the [3Fe-4S]+/0 cluster. Biochemistry,38, 8228-8239. PDB codes: 6fdr 7fd1 7fdr 10353811 M.P.Golinelli, N.H.Chmiel, and S.S.David (1999). Site-directed mutagenesis of the cysteine ligands to the [4Fe-4S] cluster of Escherichia coli MutY. Biochemistry,38, 6997-7007. 9737860 M.A.Kemper, H.S.Gao-Sheridan, B.Shen, J.L.Duff, G.J.Tilley, F.A.Armstrong, and B.K.Burgess (1998). Delta T 14/Delta D 15 Azotobacter vinelandii ferredoxin I: creation of a new CysXXCysXXCys motif that ligates a [4Fe-4S] cluster. Biochemistry,37, 12829-12837. 9671512 M.P.Golinelli, C.Chatelet, E.C.Duin, M.K.Johnson, and J.Meyer (1998). Extensive ligand rearrangements around the [2Fe-2S] cluster of Clostridium pasteurianum ferredoxin. Biochemistry,37, 10429-10437. 9657695 S.Aono, D.Bentrop, I.Bertini, A.Donaire, C.Luchinat, Y.Niikura, and A.Rosato (1998). Solution structure of the oxidized Fe7S8 ferredoxin from the thermophilic bacterium Bacillus schlegelii by 1H NMR spectroscopy. Biochemistry,37, 9812-9826. PDB codes: 1bc6 1bd6 9698385 W.N.Lanzilotta, J.Christiansen, D.R.Dean, and L.C.Seefeldt (1998). Evidence for coupled electron and proton transfer in the [8Fe-7S] cluster of nitrogenase. Biochemistry,37, 11376-11384. 8688437 M.P.Golinelli, L.A.Akin, B.R.Crouse, M.K.Johnson, and J.Meyer (1996). Cysteine ligand swapping on a deletable loop of the [2Fe-2S] ferredoxin from Clostridium pasteurianum. Biochemistry,35, 8995-9002. The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.