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		PDBsum entry 1rgv Go to PDB code: Electron transport PDB id 1rgv Loading ... Contents Protein chain 80 a.a.* Ligands SF4 ×2 * Residue conservation analysis PDB id: 1rgv Links PDBe RCSB MMDB JenaLib Proteopedia CATH SCOP PDBSWS PDBePISA ProSAT Name: Electron transport Title: Crystal structure of the ferredoxin from thauera aromatica Structure: Ferredoxin. Chain: a Source: Thauera aromatica. Organism_taxid: 44139. Strain: k172 Resolution: 2.90Å R-factor: 0.197 R-free: 0.219 Authors: M.Unciuleac,M.Boll,E.Warkentin,U.Ermler Key ref: M.Unciuleac et al. (2004). Crystallization of 4-hydroxybenzoyl-CoA reductase and the structure of its electron donor ferredoxin.Acta Crystallogr D Biol Crystallogr,60, 388-391.PubMed id: 14747735 DOI: 10.1107/S0907444903028506 Date: 13-Nov-03 Release date: 10-Feb-04 PROCHECK Headers References Protein chain ? O88151 (O88151_THAAR) - Ferredoxin from Thauera aromatica Seq:Struc: 81 a.a. 80 a.a. Key: PfamA domain Secondary structure CATH domain DOI no: 10.1107/S0907444903028506 Acta Crystallogr D Biol Crystallogr 60:388-391 (2004) PubMed id: 14747735 Crystallization of 4-hydroxybenzoyl-CoA reductase and the structure of its electron donor ferredoxin. M.Unciuleac, M.Boll, E.Warkentin, U.Ermler. ABSTRACT 4-Hydroxybenzoyl-CoA reductase (4-HBCR) is a central enzyme in the metabolism of phenolic compounds in anaerobic bacteria. The enzyme catalyzes the reductive removal of the phenolic hydroxyl group from 4-hydroxybenzoyl-CoA, yielding benzoyl-CoA and water. 4-HBCR belongs to the xanthine oxidase (XO) family of molybdenum enzymes which occur as heterodimers, (alphabetagamma)(2). 4-HBCR clusters and two FADs. A low-potential Allochromatium vinosum-type ferredoxin containing two clusters serves as an in vivo electron donor for 4-HBCR. In this work, the oxygen-sensitive proteins 4-HBCR and the ferredoxin (TaFd) from Thauera aromatica were crystallized under anaerobic conditions. 4-HBCR crystallized with PEG 4000 and MPD as precipitant diffracted to about 1.6 A resolution and the crystals were highly suitable for X-ray structure analysis. Crystals of TaFd were obtained with (NH(4))(3)PO(4) as precipitant and revealed a solvent content of 77%, which is remarkably high for a small soluble protein. The structure of TaFd was solved at 2.9 A resolution by the molecular-replacement method using the highly related structure of the ferredoxin (CvFd) from A. vinosum as a cluster can be correlated with their different redox potentials. Selected figure(s) Figure 1. Figure 1 Reactions catalyzed by benzoyl-CoA reductase (1) and 4-hydroxybenzoyl-CoA reductase (2). In T. aromatica, reduced ferredoxin serves as in vivo electron donor for both reactions; it is regenerated by 2-oxoglutarate:ferredoxin oxidoreductase. The above figure is reprinted by permission from the IUCr: Acta Crystallogr D Biol Crystallogr (2004,60, 388-391) copyright 2004. Figure was selected by an automated process. Literature references that cite this PDB file's key reference PubMed id Reference 17457690 M.Heinnickel, and J.H.Golbeck (2007). Heliobacterial photosynthesis. Photosynth Res,92, 35-53. 16596388 P.Giastas, N.Pinotsis, G.Efthymiou, M.Wilmanns, P.Kyritsis, J.M.Moulis, and I.M.Mavridis (2006). The structure of the 2[4Fe-4S] ferredoxin from Pseudomonas aeruginosa at 1.32-A resolution: comparison with other high-resolution structures of ferredoxins and contributing structural features to reduction potential values. J Biol Inorg Chem,11, 445-458. PDB code: 2fgo 16218872 M.Boll, B.Schink, A.Messerschmidt, and P.M.Kroneck (2005). Novel bacterial molybdenum and tungsten enzymes: three-dimensional structure, spectroscopy, and reaction mechanism. Biol Chem,386, 999. 15576037 M.Unciuleac, E.Warkentin, C.C.Page, M.Boll, and U.Ermler (2004). Structure of a xanthine oxidase-related 4-hydroxybenzoyl-CoA reductase with an additional [4Fe-4S] cluster and an inverted electron flow. Structure,12, 2249-2256. PDB codes: 1rm6 1sb3 The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.