ENCBS778AGF – ENCODE (original) (raw)

1. Biosamples
2. [cell line](/search/?type=Biosample&biosample%5Fontology.classification=cell line&status=released "Search for cell line in released Biosamples")
3. [_Homo sapiens_](/search/?type=Biosample&biosample%5Fontology.classification=cell line&organism.scientific%5Fname=Homo sapiens&status=released "Search for cell line and Homo sapiens in released Biosamples")
4. [MCF-7](/search/?type=Biosample&biosample%5Fontology.classification=cell line&organism.scientific%5Fname=Homo sapiens&biosample%5Fontology.term%5Fname=MCF-7&status=released "Search for cell line and Homo sapiens and MCF-7 in released Biosamples")

ENCBS778AGF / cell line

Summary

Term name

MCF-7

Term ID

EFO:0001203

Summary

Homo sapiens MCF-7 cell line expressing RNAi

Description

MCF-7 cells treated with a siRNA knockdown against a non-specific target control, used in RNA-seq experiments.

Attribution

ENCODE3 project

Lab

Peggy Farnham, USC

Award PI

Michael Snyder, Stanford

Submitted by

Heather Witt

Source

ATCC

Project

ENCODE

Aliases

peggy-farnham:B-MCF-7-siRNA-control-rep3

Genetic modifications

Donor information

Accession

ENCDO000AAE

Aliases

encode:donor of MCF-7, bradley-bernstein:Donor of MCF-7 cells

Species

Homo sapiens

Life stage

adult

Age

69 years

Sex

female

Health status

breast cancer (adenocarcinoma)

Ethnicity

European

Documents

transfection protocol

Description: Basic transfection protoco with Dharmafectl from Dharmacon

Submitted by

Heather Witt

Lab

Peggy Farnham, USC

Grant

U54HG006996

other

Description: Basic siRNA suspension protocol from Dharmacon

Submitted by

Heather Witt

Lab

Peggy Farnham, USC

Grant

U54HG006996