original ) (raw )
domestic goat polyclonal
domestic rabbit monoclonal (7B9W3)
domestic rabbit polyclonal
mouse monoclonal (H-4)
mouse monoclonal (P5H9-A3)
TMPRSS2 (H-4) AC Santa Cruz Biotechnology 4 domestic rabbit polyclonal
TMPRSS2 HUABIO TMPRSS2 HUABIO Western blot analysis of TMPRSS2 on different lysates with Mouse anti-TMPRSS2 antibody (HA600057) at 1/1,000 dilution. Lane 1: Mouse pancreas tissue lysate Lane 2: Mouse kidney tissue lysate Lane 3: Rat pancreas tissue lysate Lane 4: Rat kidney tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 54 kDa Observed band size: 70 kDa Exposure time: 30 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600057) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. Immunocytochemistry analysis of HeLa cells labeling TMPRSS2 with Mouse anti-TMPRSS2 antibody (HA600057) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-TMPRSS2 antibody (HA600057) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. Western blot analysis of TMPRSS2 on Hela cell lysates with Mouse anti-TMPRSS2 antibody (HA600057) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 54 kDa Observed band size: 50 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600057) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature. to the supplier domestic rabbit polyclonal
mouse monoclonal (1038127)
mouse monoclonal (1038105)
human monoclonal
domestic rabbit recombinant
mouse monoclonal (S20014A)
mouse monoclonal (S20014A)
mouse monoclonal (S20014A)
domestic rabbit monoclonal
domestic rabbit monoclonal
domestic rabbit monoclonal
domestic rabbit monoclonal
domestic goat polyclonal
Western blot analysis of TMPRSS2 on different lysates with Mouse anti-TMPRSS2 antibody (HA600057) at 1/1,000 dilution. Lane 1: Mouse pancreas tissue lysate Lane 2: Mouse kidney tissue lysate Lane 3: Rat pancreas tissue lysate Lane 4: Rat kidney tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 54 kDa Observed band size: 70 kDa Exposure time: 30 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600057) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. 
Immunocytochemistry analysis of HeLa cells labeling TMPRSS2 with Mouse anti-TMPRSS2 antibody (HA600057) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-TMPRSS2 antibody (HA600057) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. 
Western blot analysis of TMPRSS2 on Hela cell lysates with Mouse anti-TMPRSS2 antibody (HA600057) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 54 kDa Observed band size: 50 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600057) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature. quantity: 100μl