mouse Pou5f1 antibody | 37 (2 knockout-validated) products in Validated Antibody Database; 58 cited in the literature; 106 total from 12 suppliers (original ) (raw )
mouse monoclonal (C-10) reactivity: African green monkey, human, mouse, rat, dogs, bovine, pigs , Xenopus laevis, domestic rabbit, rhesus macaque application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, EMSA, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, ChIP-Seq, In-Cell Western, RNA immunoprecipitation, other, western blot knockout validation citations: 714
mouse monoclonal (A-9) reactivity: human, mouse, rat application: western blot, ELISA, immunocytochemistry, immunoprecipitation citations: 6
mouse monoclonal (C-10) reactivity: human, mouse, rat application: western blot, immunohistochemistry, immunocytochemistry, other citations: 31
domestic rabbit polyclonal reactivity: domestic goat, human, mouse, pigs , domestic horse application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, ChIP-Seq, western blot knockout validation citations: 110
domestic rabbit polyclonal reactivity: human, mouse, rat application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section citations: 23
mouse monoclonal (GT486) reactivity: human, mouse application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section citations: 16
domestic rabbit polyclonal reactivity: human, mouse application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section citations: 14
rat monoclonal (EM92) reactivity: human, mouse conjugate: efluor 660 application: flow cytometry citations: 12
mouse monoclonal (9B7) reactivity: human, mouse application: western blot, immunocytochemistry, flow cytometry citations: 11 Flow cytometry analysis of Oct4 (red histogram) on human NCCIT cells. Cells were harvested, fixed with 4% formaldehyde, washed with PBS, and incubated with an Oct4 monoclonal antibody (Product # MA1-104) at a 1:100 dilution or PBS alone (blue histogram) for 1 hour on ice. For flow analysis, a 30-minute incubation with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) was performed and 10,000 cells were acquired for each sample. Western blot analysis of Oct4 was performed by loading 75 ug of the indicated whole cell lysates and 5ul of the Lane Marker Reducing Sample Buffer (Product # 39000) per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST (Product # 37525) for at least 1 hour. The membrane was probed with an Oct4 monoclonal antibody (Product # MA1-104) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product # 32430) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075). **Note: the antibody is detecting Oct4 in the human embryonal carcinoma cells, but not in negative control HeLa cells. Unknown bands at 80kDa were also observed with this antibody. Immunofluorescent analysis of Oct4 (green) in retinoic acid-treated NCCIT cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 0.3% BSA/TBST (Product # 37525) for 15 minutes at room temperature. Cells were probed with an Oct4 monoclonal antibody (Product # MA1-104) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification. **Note: the absence of Oct4 staining in a subset of the NCCIT cells suggests differentiation. In addition to the nuclear staining of Oct4, the antibody also shows some cytoplasmic staining. quantity: 100 µg price: US 390.00to the supplier
rat monoclonal (240408) reactivity: human, mouse application: western blot, immunocytochemistry citations: 39
domestic goat polyclonal reactivity: human, mouse application: western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation citations: 31
rat monoclonal (240408) reactivity: human, mouse conjugate: PE application: flow cytometry citations: 10
domestic rabbit polyclonal reactivity: human, mouse, rat, bovine application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section citations: 53 quantity: 0.1 ml (also 0.025 ml) price: 399 USDto the supplier
mouse monoclonal (486) reactivity: human, mouse application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section citations: 1
mouse monoclonal (OTI7H1) reactivity: human, mouse, rat application: western blot, immunocytochemistry
mouse monoclonal (3A2A20) reactivity: human, mouse conjugate: AF488 application: immunocytochemistry, flow cytometry citations: 11
mouse monoclonal (3A2A20) reactivity: human, mouse application: immunocytochemistry citations: 10
mouse monoclonal (3A2A20) reactivity: human, mouse application: western blot, immunocytochemistry, chromatin immunoprecipitation citations: 8
mouse monoclonal reactivity: mouse application: western blot, ELISA
domestic rabbit polyclonal reactivity: human, mouse, dogs, bovine, chimpanzee application: western blot, ELISA, immunocytochemistry, immunoprecipitation
domestic rabbit polyclonal reactivity: human, mouse, bovine application: western blot, immunocytochemistry, flow cytometry
mouse monoclonal (3F5-F9-E5) reactivity: mouse application: western blot
Oct-4 Antibody Rockland Immunochemicals catalog: 600-401-A95 domestic rabbit polyclonal reactivity: human, mouse application: western blot, ELISA, immunoprecipitation
domestic rabbit polyclonal reactivity: human, mouse, rat application: western blot, ELISA, immunohistochemistry
domestic rabbit polyclonal reactivity: human, mouse application: western blot, ELISA, immunohistochemistry
domestic goat polyclonal reactivity: human, mouse, rat, dogs application: western blot, enzyme immunoassay
domestic rabbit polyclonal reactivity: human, mouse application: western blot, immunohistochemistry
domestic rabbit monoclonal (C30A3) reactivity: human, mouse application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section citations: 47
Oct-3/4 BD Biosciences catalog: 611202 total number of products: 9 mouse monoclonal (40/Oct-3) reactivity: human, mouse application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section citations: 22