Nine Amino Acids Transactivation Domain 9aaTAD Prediction Tool (original) (raw)

Dipl. Ing. Martin Piskáček, Dr. rer. nat.

Sequence:

Choose a recommended basic search pattern:

Moderately stringent Pattern
(generally best for mammals and yeast Transcription Factors)
[MDENQSTYG]{KRHCGP}[ILVFWM]{KRHCGP}{CGP}{CGP}[ILVFWM]{CGP}{CGP}

Most stringent Pattern
(best for most yeast Transcription Factors)
MDENQSTYG]{KRHCGP}[ILVFWM]{KRHCGP}{CGP}{KRHCGP}[ILVFWM][ILVFWMAY]{KRHC}

Less stringent Pattern
(best for viral, artificial and other Transcription Factors or short Transactivation Domains w/o suitable hits with stringent Patterns)
[MDENQSTYCPGA]X[ILVFWMAY]{KRHCGP}{CGP}{CGP}[ILVFWMAY]XX

Pattern for Clusters of less stringent DFx Repeats
(best for short Transactivation Domains w/o suitable hits with stringent Patterns)
XX[ILVFWMAY]{GP}{GP}[ILVFWMAY]{GP}XX

Custom:

Refinement criteria:

| | Approved positions | | | | | | | | | | -------------------- | -------------------------------- | - | - | - | - | - | - | - | | | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | | RC1 | min 1 x [ILVFWMAY] | | | | | | | | | RC2 | min 1 x [ILVFWMAY] | | | | | | | | | RC3 | min 1 x [DENQST] | | | | | | | | | RC4 | min 2 - max 4 x [DENQSTKRH] | | | | | | | | | RC5 | min 5 - max 7 x [ILVFWMAYGPST] | | | | | | | | | RC6 | max 3 x [ILVFWMAYGP] in a row | | | | | | | | | RC7 | max 2 x [DENQST] in a row | | | | | | | | | RC8 | max 1 x [KRH] | | | | | | | | | RC9 | max 1 x [QNKRH] | | | | | | | | | RC10 | max 1 x [QNKRH] | | | | | | | | | RC11 | any [C] | | | | | | | | | | RC12 | any [PG] | | | | | | | |