Detailed deletion mapping of chromosome band 14q32 in human neuroblastoma defines a 1.1-Mb region of common allelic loss (original) (raw)

Abstract

Neuroblastoma (NB) is a well-known malignant disease in infants, but its molecular mechanisms have not yet been fully elucidated. To investigate the genetic contribution of abnormalities on the long arm of chromosome 14 (14q) in NB, we analysed loss of heterozygosity (LOH) in 54 primary NB samples using 12 microsatellite markers on 14q32. Seventeen (31%) of 54 tumours showed LOH at one or more of the markers analysed, and the smallest common region of allelic loss was identified between D14S62 and D14S987. This region was estimated to be 1-cM long from the linkage map. Fluorescence in situ hybridization also confirmed the loss. There was no statistical correlation between LOH and any clinicopathologic features, including age, stage, amplification of MYCN and ploidy. We further constructed a contig spanning the lost region using bacterial artificial chromosome and estimated this region to be approximately 1.1-Mb by pulsed-field gel electrophoresis. Our results will contribute to cloning and characterizing the putative tumour-associated gene(s) in 14q32 in NB. © 2000 Cancer Research Campaign

Full Text

The Full Text of this article is available as a PDF (165.7 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ariyama T., Inazawa J., Ezaki T., Nakamura Y., Horii A., Abe T. High-resolution cytogenetic mapping of the short arm of chromosome 1 with newly isolated 411 cosmid markers by fluorescence in situ hybridization: the precise order of 18 markers on 1p36.1 on prophase chromosomes and "stretched" DNAs. Genomics. 1995 Jan 1;25(1):114–123. doi: 10.1016/0888-7543(95)80116-4. [DOI] [PubMed] [Google Scholar]
  2. Asakawa S., Abe I., Kudoh Y., Kishi N., Wang Y., Kubota R., Kudoh J., Kawasaki K., Minoshima S., Shimizu N. Human BAC library: construction and rapid screening. Gene. 1997 May 20;191(1):69–79. doi: 10.1016/s0378-1119(97)00044-9. [DOI] [PubMed] [Google Scholar]
  3. Bandera C. A., Takahashi H., Behbakht K., Liu P. C., LiVolsi V. A., Benjamin I., Morgan M. A., King S. A., Rubin S. C., Boyd J. Deletion mapping of two potential chromosome 14 tumor suppressor gene loci in ovarian carcinoma. Cancer Res. 1997 Feb 1;57(3):513–515. [PubMed] [Google Scholar]
  4. Bown N., Cotterill S., Lastowska M., O'Neill S., Pearson A. D., Plantaz D., Meddeb M., Danglot G., Brinkschmidt C., Christiansen H. Gain of chromosome arm 17q and adverse outcome in patients with neuroblastoma. N Engl J Med. 1999 Jun 24;340(25):1954–1961. doi: 10.1056/NEJM199906243402504. [DOI] [PubMed] [Google Scholar]
  5. Brodeur G. M., Azar C., Brother M., Hiemstra J., Kaufman B., Marshall H., Moley J., Nakagawara A., Saylors R., Scavarda N. Neuroblastoma. Effect of genetic factors on prognosis and treatment. Cancer. 1992 Sep 15;70(6 Suppl):1685–1694. doi: 10.1002/1097-0142(19920915)70:4+<1685::aid-cncr2820701607>3.0.co;2-h. [DOI] [PubMed] [Google Scholar]
  6. Brodeur G. M., Green A. A., Hayes F. A., Williams K. J., Williams D. L., Tsiatis A. A. Cytogenetic features of human neuroblastomas and cell lines. Cancer Res. 1981 Nov;41(11 Pt 1):4678–4686. [PubMed] [Google Scholar]
  7. Caron H., Peter M., van Sluis P., Speleman F., de Kraker J., Laureys G., Michon J., Brugières L., Voûte P. A., Westerveld A. Evidence for two tumour suppressor loci on chromosomal bands 1p35-36 involved in neuroblastoma: one probably imprinted, another associated with N-myc amplification. Hum Mol Genet. 1995 Apr;4(4):535–539. doi: 10.1093/hmg/4.4.535. [DOI] [PubMed] [Google Scholar]
  8. Caron H., van Sluis P., de Kraker J., Bökkerink J., Egeler M., Laureys G., Slater R., Westerveld A., Voûte P. A., Versteeg R. Allelic loss of chromosome 1p as a predictor of unfavorable outcome in patients with neuroblastoma. N Engl J Med. 1996 Jan 25;334(4):225–230. doi: 10.1056/NEJM199601253340404. [DOI] [PubMed] [Google Scholar]
  9. Caron H., van Sluis P., van Hoeve M., de Kraker J., Bras J., Slater R., Mannens M., Voûte P. A., Westerveld A., Versteeg R. Allelic loss of chromosome 1p36 in neuroblastoma is of preferential maternal origin and correlates with N-myc amplification. Nat Genet. 1993 Jun;4(2):187–190. doi: 10.1038/ng0693-187. [DOI] [PubMed] [Google Scholar]
  10. Cheng N. C., Van Roy N., Chan A., Beitsma M., Westerveld A., Speleman F., Versteeg R. Deletion mapping in neuroblastoma cell lines suggests two distinct tumor suppressor genes in the 1p35-36 region, only one of which is associated with N-myc amplification. Oncogene. 1995 Jan 19;10(2):291–297. [PubMed] [Google Scholar]
  11. Cox D. W., Billingsley G., Nguyen V. T. A linkage map of human chromosome 14, including 13 gene loci. Genomics. 1994 Sep 15;23(2):331–337. doi: 10.1006/geno.1994.1508. [DOI] [PubMed] [Google Scholar]
  12. Cox D. W. Report of the first international workshop on human chromosome 14 mapping 1993. Cytogenet Cell Genet. 1994;66(1):2–9. [PubMed] [Google Scholar]
  13. Dib C., Fauré S., Fizames C., Samson D., Drouot N., Vignal A., Millasseau P., Marc S., Hazan J., Seboun E. A comprehensive genetic map of the human genome based on 5,264 microsatellites. Nature. 1996 Mar 14;380(6570):152–154. doi: 10.1038/380152a0. [DOI] [PubMed] [Google Scholar]
  14. Evans A. E., D'Angio G. J., Randolph J. A proposed staging for children with neuroblastoma. Children's cancer study group A. Cancer. 1971 Feb;27(2):374–378. doi: 10.1002/1097-0142(197102)27:2<374::aid-cncr2820270221>3.0.co;2-g. [DOI] [PubMed] [Google Scholar]
  15. Fong C. T., Dracopoli N. C., White P. S., Merrill P. T., Griffith R. C., Housman D. E., Brodeur G. M. Loss of heterozygosity for the short arm of chromosome 1 in human neuroblastomas: correlation with N-myc amplification. Proc Natl Acad Sci U S A. 1989 May;86(10):3753–3757. doi: 10.1073/pnas.86.10.3753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Fong C. T., White P. S., Peterson K., Sapienza C., Cavenee W. K., Kern S. E., Vogelstein B., Cantor A. B., Look A. T., Brodeur G. M. Loss of heterozygosity for chromosomes 1 or 14 defines subsets of advanced neuroblastomas. Cancer Res. 1992 Apr 1;52(7):1780–1785. [PubMed] [Google Scholar]
  17. Gilbert F., Feder M., Balaban G., Brangman D., Lurie D. K., Podolsky R., Rinaldt V., Vinikoor N., Weisband J. Human neuroblastomas and abnormalities of chromosomes 1 and 17. Cancer Res. 1984 Nov;44(11):5444–5449. [PubMed] [Google Scholar]
  18. Gyapay G., Morissette J., Vignal A., Dib C., Fizames C., Millasseau P., Marc S., Bernardi G., Lathrop M., Weissenbach J. The 1993-94 Généthon human genetic linkage map. Nat Genet. 1994 Jun;7(2 Spec No):246–339. doi: 10.1038/ng0694supp-246. [DOI] [PubMed] [Google Scholar]
  19. Hofker M. H., Smith S., Nakamura Y., Teshima I., White R., Cox D. W. Physical mapping of probes within 14q32, a subtelomeric region showing a high recombination frequency. Genomics. 1990 Jan;6(1):33–38. doi: 10.1016/0888-7543(90)90445-z. [DOI] [PubMed] [Google Scholar]
  20. Kafatos F. C., Jones C. W., Efstratiadis A. Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure. Nucleic Acids Res. 1979 Nov 24;7(6):1541–1552. doi: 10.1093/nar/7.6.1541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Kimura M., Abe T., Sunamura M., Matsuno S., Horii A. Detailed deletion mapping on chromosome arm 12q in human pancreatic adenocarcinoma: identification of a I-cM region of common allelic loss. Genes Chromosomes Cancer. 1996 Oct;17(2):88–93. doi: 10.1002/(SICI)1098-2264(199610)17:2<88::AID-GCC3>3.0.CO;2-X. [DOI] [PubMed] [Google Scholar]
  22. Kovacs G., Frisch S. Clonal chromosome abnormalities in tumor cells from patients with sporadic renal cell carcinomas. Cancer Res. 1989 Feb 1;49(3):651–659. [PubMed] [Google Scholar]
  23. Laureys G., Speleman F., Versteeg R., van der Drift P., Chan A., Leroy J., Francke U., Opdenakker G., Van Roy N. Constitutional translocation t(1;17)(p36.31-p36.13;q11.2-q12.1) in a neuroblastoma patient. Establishment of somatic cell hybrids and identification of PND/A12M2 on chromosome 1 and NF1/SCYA7 on chromosome 17 as breakpoint flanking single copy markers. Oncogene. 1995 Mar 16;10(6):1087–1093. [PubMed] [Google Scholar]
  24. Martinsson T., Sjöberg R. M., Hedborg F., Kogner P. Deletion of chromosome 1p loci and microsatellite instability in neuroblastomas analyzed with short-tandem repeat polymorphisms. Cancer Res. 1995 Dec 1;55(23):5681–5686. [PubMed] [Google Scholar]
  25. Martinsson T., Weith A., Cziepluch C., Schwab M. Chromosome 1 deletions in human neuroblastomas: generation and fine mapping of microclones from the distal 1p region. Genes Chromosomes Cancer. 1989 Sep;1(1):67–78. doi: 10.1002/gcc.2870010111. [DOI] [PubMed] [Google Scholar]
  26. Nagase S., Yamakawa H., Sato S., Yajima A., Horii A. Identification of a 790-kilobase region of common allelic loss in chromosome 10q25-q26 in human endometrial cancer. Cancer Res. 1997 May 1;57(9):1630–1633. [PubMed] [Google Scholar]
  27. Sasaki M., Okamoto M., Sato C., Sugio K., Soejima J., Iwama T., Ikeuchi T., Tonomura A., Miyaki M., Sasazuki T. Loss of constitutional heterozygosity in colorectal tumors from patients with familial polyposis coli and those with nonpolyposis colorectal carcinoma. Cancer Res. 1989 Aug 15;49(16):4402–4406. [PubMed] [Google Scholar]
  28. Sato T., Tanigami A., Yamakawa K., Akiyama F., Kasumi F., Sakamoto G., Nakamura Y. Allelotype of breast cancer: cumulative allele losses promote tumor progression in primary breast cancer. Cancer Res. 1990 Nov 15;50(22):7184–7189. [PubMed] [Google Scholar]
  29. Schleiermacher G., Peter M., Michon J., Hugot J. P., Vielh P., Zucker J. M., Magdelénat H., Thomas G., Delattre O. Two distinct deleted regions on the short arm of chromosome 1 in neuroblastoma. Genes Chromosomes Cancer. 1994 Aug;10(4):275–281. doi: 10.1002/gcc.2870100409. [DOI] [PubMed] [Google Scholar]
  30. Shizuya H., Birren B., Kim U. J., Mancino V., Slepak T., Tachiiri Y., Simon M. Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector. Proc Natl Acad Sci U S A. 1992 Sep 15;89(18):8794–8797. doi: 10.1073/pnas.89.18.8794. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Srivatsan E. S., Ying K. L., Seeger R. C. Deletion of chromosome 11 and of 14q sequences in neuroblastoma. Genes Chromosomes Cancer. 1993 May;7(1):32–37. doi: 10.1002/gcc.2870070106. [DOI] [PubMed] [Google Scholar]
  32. Suzuki T., Yokota J., Mugishima H., Okabe I., Ookuni M., Sugimura T., Terada M. Frequent loss of heterozygosity on chromosome 14q in neuroblastoma. Cancer Res. 1989 Mar 1;49(5):1095–1098. [PubMed] [Google Scholar]
  33. Takayama H., Suzuki T., Mugishima H., Fujisawa T., Ookuni M., Schwab M., Gehring M., Nakamura Y., Sugimura T., Terada M. Deletion mapping of chromosomes 14q and 1p in human neuroblastoma. Oncogene. 1992 Jun;7(6):1185–1189. [PubMed] [Google Scholar]
  34. Takeda O., Homma C., Maseki N., Sakurai M., Kanda N., Schwab M., Nakamura Y., Kaneko Y. There may be two tumor suppressor genes on chromosome arm 1p closely associated with biologically distinct subtypes of neuroblastoma. Genes Chromosomes Cancer. 1994 May;10(1):30–39. doi: 10.1002/gcc.2870100106. [DOI] [PubMed] [Google Scholar]
  35. Theobald M., Christiansen H., Schmidt A., Melekian B., Wolkewitz N., Christiansen N. M., Brinkschmidt C., Berthold F., Lampert F. Sublocalization of putative tumor suppressor gene loci on chromosome arm 14q in neuroblastoma. Genes Chromosomes Cancer. 1999 Sep;26(1):40–46. [PubMed] [Google Scholar]
  36. Weith A., Martinsson T., Cziepluch C., Brüderlein S., Amler L. C., Berthold F., Schwab M. Neuroblastoma consensus deletion maps to 1p36.1-2. Genes Chromosomes Cancer. 1989 Nov;1(2):159–166. doi: 10.1002/gcc.2870010209. [DOI] [PubMed] [Google Scholar]
  37. White P. S., Maris J. M., Beltinger C., Sulman E., Marshall H. N., Fujimori M., Kaufman B. A., Biegel J. A., Allen C., Hilliard C. A region of consistent deletion in neuroblastoma maps within human chromosome 1p36.2-36.3. Proc Natl Acad Sci U S A. 1995 Jun 6;92(12):5520–5524. doi: 10.1073/pnas.92.12.5520. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Young J., Leggett B., Ward M., Thomas L., Buttenshaw R., Searle J., Chenevix-Trench G. Frequent loss of heterozygosity on chromosome 14 occurs in advanced colorectal carcinomas. Oncogene. 1993 Mar;8(3):671–675. [PubMed] [Google Scholar]