Renal mesangial cell cultures as a model for study of erythropoietin production (original) (raw)

Abstract

Mesangial cells derived from isolated glomeruli of rat kidney were grown as homogeneous cell lines in culture. They released, into the culture medium, erythropoietin that had free terminal galactosyl residues and was therefore not active in vivo. The production of erythropoietin by these cells was significantly enhanced by either lowering the PO2 in the incubation atmosphere or by adding cobalt chloride to the culture medium. Therefore, mesangial cells in culture may be considered as an in vitro system in which the regulation of erythropoietin production can be studied under well-defined conditions.

4008

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ausiello D. A., Kreisberg J. I., Roy C., Karnovsky M. J. Contraction of cultured rat glomerular cells of apparent mesangial origin after stimulation with angiotensin II and arginine vasopressin. J Clin Invest. 1980 Mar;65(3):754–760. doi: 10.1172/JCI109723. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bachmann S., Kriz W., Kuhn C., Franke W. W. Differentiation of cell types in the mammalian kidney by immunofluorescence microscopy using antibodies to intermediate filament proteins and desmoplakins. Histochemistry. 1983;77(3):365–394. doi: 10.1007/BF00490899. [DOI] [PubMed] [Google Scholar]
  3. Barajas L. Anatomy of the juxtaglomerular apparatus. Am J Physiol. 1979 Nov;237(5):F333–F343. doi: 10.1152/ajprenal.1979.237.5.F333. [DOI] [PubMed] [Google Scholar]
  4. Bennett G., O'Shaughnessy D. The site of incorporation of sialic acid residues into glycoproteins and the subsequent fates of these molecules in various rat and mouse cell types as shown by radioautography after injection of [3H]N-acetylmannosamine. I. Observations in hepatocytes. J Cell Biol. 1981 Jan;88(1):1–15. doi: 10.1083/jcb.88.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Burkholder P. M. Functions and pathophysiology of the glomerular mesangium. Lab Invest. 1982 Feb;46(2):239–241. [PubMed] [Google Scholar]
  6. Burlington H., Cronkite E. P., Reincke U., Zanjani E. D. Erythropoietin production in cultures of goat renal glomeruli. Proc Natl Acad Sci U S A. 1972 Dec;69(12):3547–3550. doi: 10.1073/pnas.69.12.3547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. COTES P. M., BANGHAM D. R. Bio-assay of erythropoietin in mice made polycythaemic by exposure to air at a reduced pressure. Nature. 1961 Sep 9;191:1065–1067. doi: 10.1038/1911065a0. [DOI] [PubMed] [Google Scholar]
  8. Erslev A. J., Caro J., Kansu E., Silver R. Renal and extrarenal erythropoietin production in anaemic rats. Br J Haematol. 1980 May;45(1):65–72. doi: 10.1111/j.1365-2141.1980.tb03811.x. [DOI] [PubMed] [Google Scholar]
  9. Foidart J. B., Dechenne C. A., Mahieu P., Creutz C. E., de Mey J. Tissue culture of normal rat glomeruli. Isolation and morphological characterization of two homogeneous cell lines. Invest Cell Pathol. 1979 Jan-Mar;2(1):15–26. [PubMed] [Google Scholar]
  10. GOLDWASSER E., JACOBSON L. O., FRIED W., PLZAK L. F. Studies on erythropoiesis. V. The effect of cobalt on the production of erythropoietin. Blood. 1958 Jan;13(1):55–60. [PubMed] [Google Scholar]
  11. GOLDWASSER E., WHITE W. F., TAYLOR K. B. Further purification of sheep plasma erythropoietin. Biochim Biophys Acta. 1962 Nov 5;64:487–496. doi: 10.1016/0006-3002(62)90306-2. [DOI] [PubMed] [Google Scholar]
  12. Gilbert S. F., Migeon B. R. D-valine as a selective agent for normal human and rodent epithelial cells in culture. Cell. 1975 May;5(1):11–17. doi: 10.1016/0092-8674(75)90086-0. [DOI] [PubMed] [Google Scholar]
  13. Goldwasser E., Kung C. K., Eliason J. On the mechanism of erythropoietin-induced differentiation. 13. The role of sialic acid in erythropoietin action. J Biol Chem. 1974 Jul 10;249(13):4202–4206. [PubMed] [Google Scholar]
  14. Gruber D. F., Zucali J. R., Wleklinski J., LaRussa V., Mirand E. A. Temporal transition in the site of rat erythropoietin production. Exp Hematol. 1977 Sep;5(5):399–407. [PubMed] [Google Scholar]
  15. Iscove N. N., Guilbert L. J., Weyman C. Complete replacement of serum in primary cultures of erythropoietin-dependent red cell precursors (CFU-E) by albumin, transferrin, iron, unsaturated fatty acid, lecithin and cholesterol. Exp Cell Res. 1980 Mar;126(1):121–126. doi: 10.1016/0014-4827(80)90476-0. [DOI] [PubMed] [Google Scholar]
  16. Jelkmann W., Bauer C. Demonstration of high levels of erythropoietin in rat kidneys following hypoxic hypoxia. Pflugers Arch. 1981 Nov;392(1):34–39. doi: 10.1007/BF00584579. [DOI] [PubMed] [Google Scholar]
  17. Jelkmann W., Brookins J., Fisher J. W. Indomethacin blockade of albuterol-induced erythropoietin production in isolated perfused dog kidneys. Proc Soc Exp Biol Med. 1979 Oct;162(1):65–70. doi: 10.3181/00379727-162-40619. [DOI] [PubMed] [Google Scholar]
  18. Kalden J. R., Peter H. H., Roubin R., Cesarini J. P. Human peripheral Null lymphocytes. I. Isolation, immunological and functional characterization. Eur J Immunol. 1977 Aug;7(8):537–543. doi: 10.1002/eji.1830070809. [DOI] [PubMed] [Google Scholar]
  19. Kreisberg J. I., Hoover R. L., Karnovsky M. J. Isolation and characterization of rat glomerular epithelial cells in vitro. Kidney Int. 1978 Jul;14(1):21–30. doi: 10.1038/ki.1978.86. [DOI] [PubMed] [Google Scholar]
  20. Kurtz A., Jelkmann W., Bauer C. Insulin stimulates erythroid colony formation independently of erythropoietin. Br J Haematol. 1983 Feb;53(2):311–316. doi: 10.1111/j.1365-2141.1983.tb02025.x. [DOI] [PubMed] [Google Scholar]
  21. Kurtz A., Jelkmann W., Bauer C. Mesangial cells derived from rat glomeruli produce an erythropoiesis stimulating factor in cell culture. FEBS Lett. 1982 Jan 11;137(1):129–132. doi: 10.1016/0014-5793(82)80330-x. [DOI] [PubMed] [Google Scholar]
  22. Lukowsky W. A., Painter R. H. Studies on the role of sialic acid in the physical and biological properties of erythropoietin. Can J Biochem. 1972 Aug;50(8):909–917. doi: 10.1139/o72-127. [DOI] [PubMed] [Google Scholar]
  23. Mahieu P. R., Foidart J. B., Dubois C. H., Dechenne C. A., Deheneffe J. Tissue culture of normal rat glomeruli: contractile activity of the cultured mesangial cells. Invest Cell Pathol. 1980 Apr-Jun;3(2):121–128. [PubMed] [Google Scholar]
  24. McCully K. S., Rinehimer L. A., Gillies C. G., Hopfer S. M., Sunderman F. W., Jr Erythrocytosis, glomerulomegaly, mesangial hyperplasia, sialyl hyperplasia, and arteriosclerosis induced in rats by nickel subsulfide. Virchows Arch A Pathol Anat Histol. 1982;394(3):207–220. doi: 10.1007/BF00430666. [DOI] [PubMed] [Google Scholar]
  25. Ogle J. W., Lange R. D., Dunn C. D. Production of erythropoietin in vitro: a review. In Vitro. 1978 Nov;14(11):945–950. doi: 10.1007/BF02616125. [DOI] [PubMed] [Google Scholar]
  26. Osborn M., Geisler N., Shaw G., Sharp G., Weber K. Intermediate filaments. Cold Spring Harb Symp Quant Biol. 1982;46(Pt 1):413–429. doi: 10.1101/sqb.1982.046.01.040. [DOI] [PubMed] [Google Scholar]
  27. Osborn M., Weber K. Immunofluorescence and immunocytochemical procedures with affinity purified antibodies: tubulin-containing structures. Methods Cell Biol. 1982;24:97–132. doi: 10.1016/s0091-679x(08)60650-0. [DOI] [PubMed] [Google Scholar]
  28. Rege A. B., Brookins J., Fisher J. W. A radioimmunoassay for erythropoietin: serum levels in normal human subjects and patients with hemopoietic disorders. J Lab Clin Med. 1982 Dec;100(6):829–843. [PubMed] [Google Scholar]
  29. Rich I. N., Kubanek B. The ontogeny of erythropoiesis in the mouse detected by the erythroid colony-forming technique. II. Transition in erythropoietin sensitivity during development. J Embryol Exp Morphol. 1980 Aug;58:143–155. [PubMed] [Google Scholar]
  30. Spivak J. L., Small D., Shaper J. H., Hollenberg M. D. Use of immobilized lectins and other ligands for the partial purification of erythropoietin. Blood. 1978 Dec;52(6):1178–1188. [PubMed] [Google Scholar]
  31. Spurr A. R. A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res. 1969 Jan;26(1):31–43. doi: 10.1016/s0022-5320(69)90033-1. [DOI] [PubMed] [Google Scholar]
  32. Travo P., Weber K., Osborn M. Co-existence of vimentin and desmin type intermediate filaments in a subpopulation of adult rat vascular smooth muscle cells growing in primary culture. Exp Cell Res. 1982 May;139(1):87–94. doi: 10.1016/0014-4827(82)90321-4. [DOI] [PubMed] [Google Scholar]