Structural and immunological characteristics of a 28-kilodalton cruzipain-like cysteine protease of Paragonimus westermani expressed in the definitive host stage - PubMed (original) (raw)

FIG. 1

Phylogenetic analysis of P. westermani 28-kDa cysteine protease by unrooted neighbor-joining tree (A) and phylogram (B). Analysis shows the position of Pw28CCP as a sister group of the S. mansoni Puerto Rican cathepsin L (branch B), which is one of the ancestral forms of the cruzipain-like cysteine proteases. The alignment set used in the analysis was 115 sites in length in the mature protein domain. The number of each node indicates the bootstrapping value obtained with 100 replicates (GenBank accession nos. are given in parentheses). 1, P. westermani 28-kDa cruzipain-like cysteine protease (Pw28CCP) (U70537); 2, P. westermani NTP (D21124); 3, P. westermani cysteine proteinase (U56865); 4, Clonorchis sinensis cysteine protease (AB020036); 5, C. sinensis cysteine protease 1 (AF093243); 6, Fasciola hepatica cathepsin L (AB009306); 7, F. hepatica secreted cathepsin L2 (U62289); 8, Fasciola sp. cysteine protease (S70380); 9, Schistosoma mansoni Puerto Rican preprocathepsin L1 (U07345); 10, S. mansoni Liberian cathepsin L (Z32529); 11, S. mansoni Liberian cathepsin C (Z32531); 12, S. mansoni cathepsin B (Sm31) (M21309); 13, Schistosoma japonicum preprocathepsin L (U38476); 14, S. japonicum preprocathepsin C (U77932); 15, S. japonicum cathepsin B (X70968); 16, Spirometra erinacei cysteine protease (D63670); 17, Ancylostoma caninum cysteine protease (AcCP-1) (U18911); 18, Caenorhabditis elegans (Bristol N2) cathepsin B (L39927); 19, Haemonchus contortus CP1 (HMCP1, Z69342); 20, HMCP2 (Z69343); 21, HMCP4 (Z69345); 22, HMCP5 (Z69346); 23, H. contortus cysteine protease (Z81327); 24, Giardia muris CP1 (AF006198); 25, G. intestinalis cysteine protease (U83277); 26, Giardia intestinalis cysteine protease (U83275); 27, Toxocara canis cathepsin Z1 (AF143817); 28, T. canis cathepsin L (U53172); 29, Trypanosoma cruzi cathepsin B (AF043246); 30, trypanosome cysteine protease (X54353); 31, T. cruzi cruzipain (M84342); 32, Trypanosoma congolense cysteine protease (L25130); 33, Leishmania donovani chagasi promastigote-specific cysteine protease (AF004593); 34, Leishmania major cathepsin L (U43706); 35, L. major cathepsin B (U43705); 36, mouse cathepsin L (M20495); 37, human cathepsin L (M20496); 38, human cathepsin B (gastric cancer, L16510); 39, human cathepsin B (kidney, M14221); 40, human cathepsin S (M90696); 41, human cathepsin S (alveolar macrophage, M86553); 42, human cathepsin X precursor (ovary) (AF073890); 43, human cathepsin X (osteoclastoma) (U20280); 44, human cathepsin C (X87212); 45, Carica papaya papain (M15203); 46, Zea mays cysteine protease (mir1) (AF019145); 47, Heteroderma glycines cathepsin L (Y09498); 48, C. papaya chymopapain (X97789); 49, Ananas comosus bromelain (D14057).