Conversion of Lactobacillus pentosus D-lactate dehydrogenase to a D-hydroxyisocaproate dehydrogenase through a single amino acid replacement - PubMed (original) (raw)

The substrate binding site of L. casei

d

-HicDH. The figure was drawn by using RasMol, according to the substrate binding site of the L. casei

d

-HicDH ternary complex structure (10). The two bound ligands, NAD+ and 2-ketoisocaproate, are shown as wire frame models. Leu51, Arg234, Glu263, and His294, which correspond to Tyr52, Arg235, Glu264, and His295 in

d

-LDHs, respectively, are indicated by ball-and-stick models.