Blood gene expression profiling of neurologic diseases: a pilot microarray study - PubMed (original) (raw)

Comparative Study

Blood gene expression profiling of neurologic diseases: a pilot microarray study

Yang Tang et al. Arch Neurol. 2005 Feb.

Abstract

Background: Tissue gene expression profiling with arrays measures the transcription of thousands of genes. However, this approach cannot be readily used to guide clinical neurologic practice.

Objectives: To determine whether clinical neurologic diseases are associated with unique patterns of up- and down-regulated genes in whole blood and to explore the possibility of using peripheral blood as a surrogate tissue in these diseases.

Design: Case-control study.

Setting: University-based pediatric and adult neurology clinics.

Participants: Patients with neurofibromatosis type 1, epilepsy, or Tourette syndrome diagnosed using traditional clinical criteria; controls without disease; and controls with neurologic disease.

Main outcome measure: Blood gene expression levels of greater than 12,000 genes, measured using U95A arrays.

Results: Neurofibromatosis type 1 and childhood epilepsy treated with carbamazepine or valproic acid are associated with distinct patterns of blood gene expression. Patients with valproic acid-responsive vs valproic acid-refractory epilepsy formed distinct subclusters. Tourette syndrome was characterized by several gene expression clusters. In 1 cluster, 6 genes-all associated with immune cell function-were overexpressed.

Conclusion: Blood gene expression profiling can provide surrogate markers for neurologic diseases without obvious blood phenotypes.

PubMed Disclaimer

Publication types

MeSH terms

Grants and funding

LinkOut - more resources