Isolation and analysis of the genes for cytochrome c oxidase in Paracoccus denitrificans - PubMed (original) (raw)

Isolation and analysis of the genes for cytochrome c oxidase in Paracoccus denitrificans

M Raitio et al. EMBO J. 1987 Sep.

Abstract

Synthetic oligonucleotide probes were used to clone two loci from the chromosomal DNA of Paracoccus denitrificans that contain the genes for cytochrome c oxidase (cytochrome aa(3)). One locus seems to contain four or five genes probably forming an operon. Two of these code for the oxidase subunits II and III. Three open reading frames are found between the COII and COIII genes. The other locus codes for the subunit I. A short open reading frame is found upstream of this gene. All three subunits of the Paracoccus enzyme show remarkable homology to the corresponding subunits of the mitochondrial cytochrome oxidase. Possible protein products of the open reading frames have not yet been identified.

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