Cell-seeding techniques in vascular tissue engineering - PubMed (original) (raw)

Review

Cell-seeding techniques in vascular tissue engineering

Gustavo A Villalona et al. Tissue Eng Part B Rev. 2010 Jun.

Abstract

Previous studies have demonstrated the benefits of cell seeding in the construction of tissue-engineered vascular grafts (TEVG). However, seeding methods are diverse and no method is clearly superior in either promoting seeding efficiency or improving long-term graft function. As we head into an era during which a variety of different TEVG are under investigation in clinical trials around the world, it is important to consider the regulatory issues surrounding the translation of these technologies. In this review, we summarize important advances in the field of vascular tissue engineering, with particular attention on cell-seeding techniques for TEVG development and special emphasis placed on regulatory issues concerning the clinical translation of these various methods.

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Figures

FIG. 1.

FIG. 1.

Static seeding. Cell suspension is pipetted directly into the lumen of the scaffold or onto the outside of the scaffold.

FIG. 2.

FIG. 2.

Rotational seeding. Scaffolds fixed to needle are placed in a spinner flask with cell suspension. The rotation of the medium within the spinner flask drives cells into the scaffold.

FIG. 3.

FIG. 3.

Vacuum/pressure seeding. A cell suspension is forced through a scaffold by either internal pressure or external vacuum pressure. As cells travel through the scaffold, they become lodged in the pores and thus seed the graft.

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