Parenteral insulin suppresses T cell proliferation to islet antigens - PubMed (original) (raw)

Randomized Controlled Trial

Parenteral insulin suppresses T cell proliferation to islet antigens

Carla J Greenbaum et al. Pediatr Diabetes. 2011 May.

Abstract

The diabetes prevention trial-type 1 (DPT-1) tested whether a combination of SQ and IV insulin therapy would delay the onset of disease in individuals at high risk of progression. We investigated whether this regimen altered T cell responses to human islet proteins using cellular immunoblotting. Among the 10 treated and 7 control subjects studied, we found that there was a significant effect of treatment on cellular immunoblotting responses. We conclude that parenteral insulin may suppress proliferation to islet antigens in individuals at risk for diabetes, but this effect may be transient. Further study is needed to determine whether a therapy that results in sustained suppression of T cell proliferation could yield a measurable clinical benefit.

© 2010 John Wiley & Sons A/S.

PubMed Disclaimer

Figures

Figure 1

Figure 1

Cellular Immunoblotting Responses in subjects enrolled in DPT-1 parenteral insulin trial. (a) Control arm subjects, n=7 (b) Treatment arm subjects, n=10. Subjects with more than 3 immunoblot sections are considered to have a positive cellular assay response. Circles indicate date of diagnosis of type 1 diabetes in 5 subjects.

References

    1. Brooks-Worrell B, Gersuk VH, Greenbaum C, Palmer JP. Intermolecular antigen spreading occurs during the preclinical period of human type 1 diabetes. J.Immunol. 2001;166:5265–5270. -PubMed
    1. Brooks-Worrell BM, Pihoker C, Greenbaum CJ, Palmer JP. Presence of islet reactive T cells and autoantibodies in children diagnosed clinically with either type 1, type 2, or atypical diabetes. 4th Immunology of Diabetes Society. 1999
    1. Brooks-Worrell BM, Starkebaum GA, Greenbaum C, Palmer JP. Peripheral blood mononuclear cells of insulin-dependent diabetic patients respond to multiple islet cell proteins. J.Immunol. 1996;157:5668–5674. -PubMed
    1. Diabetes Prevention Trial Study Group. Effects of insulin in relatives of patients with type 1 diabetes mellitus. N.Engl.J.Med. 2002;346:1685–1691. -PubMed
    1. Greenbaum CJ, Harrison LC. Guidelines for intervention trials in subjects with newly diagnosed type 1 diabetes. Diabetes. 2003;52:1059–1065. -PubMed

Publication types

MeSH terms

Substances

Grants and funding

LinkOut - more resources