Epithelial progenitor 1, a novel factor associated with epithelial cell growth and differentiation - PubMed (original) (raw)

Epithelial progenitor 1, a novel factor associated with epithelial cell growth and differentiation

Marcie R Kritzik et al. Endocrine. 2010 Apr.

Abstract

The growth and renewal of epithelial tissue is a highly orchestrated and tightly regulated process occurring in different tissue types under a variety of circumstances. We have been studying the process of pancreatic regeneration in mice. We have identified a cell surface protein, named EP1, which is expressed on the duct epithelium during pancreatic regeneration. Whereas it is not detected in the pancreas of normal mice, it is found in the intestinal epithelium of normal adult mice, as well as during pancreatic repair following cerulein-induced destruction of the acinar tissue. The distinctive situations in which EP1 is expressed, all of which share in common epithelial cell growth in the gastrointestinal tract, suggest that EP1 is involved in the growth and renewal of epithelial tissues in both the intestine and the pancreas.

PubMed Disclaimer

Figures

Fig. 1

Fig. 1

Pancreatic expression of EP1. Pancreatic sections from IFNg transgenic (a, c) and non-transgenic (b, d) mice were examined by in situ hybridization (a, b) or immunohistochemistry (c, d) for the detection of EP1 mRNA or protein, respectively. I islets, D ducts. Original magnification: ×40

Fig. 2

Fig. 2

a Intestinal expression of EP1. Tissue sections from the small intestines of wild-type (i) and IFNg transgenic (ii) mice were stained with antibody against EP1. Original magnification: panel (i), ×20; panel (ii), ×40. b FACS analysis of EP1 in intestinal epithelium. Intestinal epithelial cells were harvested from IFNg transgenic mice and stained with antibody against EP1 for FACS analysis. As indicated by the dot plot, 22% of the cells in the intestinal preparation (gated on the CD45 LCA negative population) were EP1 positive epithelial (CD49f positive) cells

Fig. 3

Fig. 3

FACS analysis of EP1 and CXCR4 expression in pancreatic epithelial cells isolated from wild-type (a) or IFNg transgenic (b) mice. Four mice were analyzed per group, and the data shown in each panel, gated on the CD45 LCA negative/CD49f positive population, is representative of one individual mouse per group

Fig. 4

Fig. 4

a panCK staining of the cerulein-treated NOD pancreas highlights the epithelial nature of the tubular structures that characterize the repair response. b–d EP1 expression in the pancreas of cerulein-treated wild-type mice (b), and cerulein-treated SDF transgenic mice (c, d). EP1 expression is indicated by the arrows in panels c and d. Original magnification: ×40

Fig. 5

Fig. 5

Sequence homology between the EP1 protein coding sequence and that of RoBo-1. The LU domains are outlined in red

References

    1. Githens S, Pediatr J. Gastroenterol. Nutr. 1988;7:486–506. -PubMed
    1. Gu D, Sarvetnick N. Development. 1993;118:33–46. -PubMed
    1. Gu D, Lee MS, Krahl T, Sarvetnick N. Development. 1994;120:1873–1881. -PubMed
    1. Kritzik MR, Krahl T, Good A, Krakowski M, St-Onge L, Gruss P, Wright C, Sarvetnick N. Mol. Cell. Endocrinol. 2000;164:99–107. -PubMed
    1. Kayali AG, Van Gunst K, Campbell IL, Stotland A, Kritzik M, Liu G, Flodstrom-Tullberg M, Zhang YQ, Sarvetnick N. J. Cell Biol. 2003;163:859–869. -PMC -PubMed

Publication types

MeSH terms

Substances

Grants and funding

LinkOut - more resources