PET Imaging and Biodistribution of Silicon Quantum Dots in Mice - PubMed (original) (raw)

PET Imaging and Biodistribution of Silicon Quantum Dots in Mice

Chuqiao Tu et al. ACS Med Chem Lett. 2011.

Abstract

Investigation of nanomaterial disposition and fate in the body is critical before such material can be translated into clinical application. Herein a new macrocyclic ligand-(64)Cu(2+) complex was synthesized and used to label dextran-coated silicon quantum dots (QD), with an average hydrodynamic diameter of 15.1 ± 7.6 nm. The chelate showed exceptional stability, demonstrated by no loss radiolabel under a ligand competition reaction with EDTA. The QDs' biodistribution in mice was quantitatively evaluated by in vivo positron emission tomography (PET) imaging and ex vivo gamma counting. Results showed that they were excreted via renal filtration shortly postinjection and also accumulated in the liver.

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Figures

Scheme 1

Scheme 1. Synthesis of Ligand 4 for 64Cu Chelation

Scheme 2

Scheme 2. Synthesis of 64Cu-DO3A Conjugated Dextran SiMn QDs 6

Figure 1

Figure 1

Stability of QDs 6-64Cu in pH 5.5 sodium acetate−acetic acid buffer solution over the 48 h time period.

Figure 2

Figure 2

Blood clearance of 64Cu-DO3A conjugated dextran SiMn QDs 6 in mice (n = 3).

Figure 3

Figure 3

In vivo PET images of mice (n = 4) at 5 min, 1 h, 4 h, 24 h, and 48 h postinjection of 64Cu-DO3A conjugated dextran SiMn QDs 6: L, liver; B, bladder.

Figure 4

Figure 4

Ex vivo biodistribution of 64Cu-DO3A conjugated dextran SiMn QDs 6 in mice. Mice (n = 4) were sacrificed 48 h post intravenous injection. Organs were harvested and measured by well gamma counting.

Figure 5

Figure 5

Hydrodynamic diameter of DO3A conjugated dextran SiMn QDs 5 measured by dynamic light scattering (DLS).

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