Tracking intracavernously injected adipose-derived stem cells to bone marrow - PubMed (original) (raw)

Tracking intracavernously injected adipose-derived stem cells to bone marrow

G Lin et al. Int J Impot Res. 2011 Nov-Dec.

Abstract

The intracavernous (i.c.) injection of stem cells (SCs) has been shown to improve erectile function in various erectile dysfunction (ED) animal models. However, the tissue distribution of the injected cells remains unknown. In this study we tracked i.c.-injected adipose-derived stem cells (ADSCs) in various tissues. Rat paratesticular fat was processed for ADSC isolation and culture. The animals were then subject to cavernous nerve (CN) crush injury or sham operation, followed by i.c. injection of 1 million autologous or allogeneic ADSCs that were labeled with 5-ethynyl-2-deoxyuridine (EdU). Another group of rats received i.c. injection of EdU-labeled allogeneic penile smooth muscle cells (PSMCs). At 2 and 7 days post injection, penises and femoral bone marrow were processed for histological analyses. Whole femoral bone marrows were also analyzed for EdU-positive cells by flow cytometry. The results show that ADSCs exited the penis within days of i.c. injection and migrated preferentially to bone marrow. Allogenicity did not affect the bone marrow appearance of ADSCs at either 2 or 7 days, whereas CN injury reduced the number of ADSCs in bone marrow significantly at 7 but not 2 days. The significance of these results in relation to SC therapy for ED is discussed.

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Conflict of interest statement

Conflict of interest: The authors declare no conflict of interest.

Figures

Fig. 1

Fig. 1

Tissue distribution of IC injected ADSCs. The indicated tissues were obtained from rats at 7 days after IC injection of one million ADSCs. All cell nuclei were stained blue by DAPI. ADSCs, which stained red by EdU, were counted manually and the results shown at the lower left corner of each tissue image.

Fig. 2

Fig. 2

Quantification of IC injected cells in penis and bone marrow. Control rats (Cont) received no injection. The other rats received IC injection of either PSMCs (PSMC) or ADSCs (ADSC), and their penis and femoral bone marrow harvested at 2 or 7 days later. Representative images of tissue sections of penis (40× magnification) and bone marrow (200× magnification) are shown in panels A and C, respectively. All cell nuclei were stained blue by DAPI. PSMCs and ADSCs, which stained red by EdU, were counted manually and the results shown in panels B and D, respectively.

Fig. 3

Fig. 3

Quantification of IC injected cells in bone marrow. Rats received IC injection of either PSMCs (PSMC) or ADSCs (ADSC), and their femoral bone marrow harvested at 2 or 7 days later for FACS analysis. Percentages shown in the FACS charts are the ratio of EdU+ cells versus total bone marrow cells (represented by 1 × 105 cells). They are further compared in the bar chart below.

Fig. 4

Fig. 4

Comparison of autologous versus allogeneic ADSCs. Rats received IC injection of either autologous or allogeneic ADSCs, and their femoral bone marrow harvested at 2 or 7 days later for histology. Representative images are shown in the upper panel. All cell nuclei were stained blue by DAPI. ADSCs, which stained red by EdU, were counted manually and the results shown in the bar chart in the lower panel.

Fig. 5

Fig. 5

Comparison of sham-treated versus CN injury rats. Sham-treated and CN injury rats received IC injection of ADSCs, and their femoral bone marrow harvested at 2 or 7 days later for histology. Representative images are shown in the upper panel. All cell nuclei were stained blue by DAPI. ADSCs, which stained red by EdU, were counted manually and the results shown in the bar chart in the lower panel.

References

    1. Huang YC, Shindel AW, Ning H, Lin G, Harraz AM, Wang G, et al. Adipose derived stem cells ameliorate hyperlipidemia associated detrusor overactivity in a rat model. J Urol. 2010;183(3):1232–1240. -PMC -PubMed
    1. Lin G, Wang G, Banie L, Ning H, Shindel AW, Fandel TM, et al. Treatment of stress urinary incontinence with adipose tissue-derived stem cells. Cytotherapy. 2010;12(1):88–95. -PMC -PubMed
    1. Ra JC, Shin IS, Kim SH, Kang SK, Kang BC, Lee HY, et al. Safety of intravenous infusion of human adipose tissue-derived mesenchymal stem cells in animals and humans. Stem Cells Dev. 2011 -PubMed
    1. Bochinski D, Lin GT, Nunes L, Carrion R, Rahman N, Lin CS, et al. The effect of neural embryonic stem cell therapy in a rat model of cavernosal nerve injury. BJU Int. 2004;94(6):904–909. -PubMed
    1. Lin CS. Advances in stem cell therapy for the lower urinary tract. world J Stem Cells. 2010;2(1):1–4. -PMC -PubMed

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