Quantitatively analyzing the protective effect of mesenchymal stem cells on cardiomyocytes in single-cell biochips - PubMed (original) (raw)

Quantitatively analyzing the protective effect of mesenchymal stem cells on cardiomyocytes in single-cell biochips

Zhen Ma et al. Biotechnol Lett. 2012 Jul.

Abstract

To understand how stem cells benefit native cardiomyocytes is crucial for cell-based therapies to rescue cardiomyocytes (CMCs) damaged during heart infarction and other cardiac diseases. However, the current conclusions on the protective effect of mesenchymal stem cells (MSCs) were obtained by analyzing the overall amount of protein and factor secretion in a conventional co-culture system. These results neglected the heterogeneity of MSC population and failed to determine the importance of cellular contact to the protective effects. To address these issues, we have constructed two biochips by microfabrication methods and laser-guided cell micropatterning technique. Using the biochips, the protective effect of MSCs on CMCs can be quantitatively analyzed at single-cell level with defined cellular contact. The role of cellular contact on protective effect can be clarified according to our statistical results.

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Figures

Fig. 1

Fig. 1

a A schematic image of laser-patterning procedure. A MSC (pink sphere) was being trapped by the laser beam and deposited into the microwell containing a CMC (green sphere); the heterotypic cell pairs in b a contact-promoting biochip and c a contact-preventing biochip right after the laser-patterning procedure; after one day culture, d two cells in a contact-promoting microwell spread and formed broad contact area, and e two cells in a contact-preventing microwell stayed in their compartments and did not contact each other

Fig. 2

Fig. 2

a α-actinin staining for CMCs and b CD90 staining for MSCs in the conventional cultures; in contact-promoting microwells, CMCs showed α-actinin positive in c homotypic CMC pair and e heterotypic cell pair, and MSCs showed CD90 positive in d homotypic MSC pair and f heterotypic cell pair; in contact-preventing microwells, a homotypic MSC pair showed CD90 positive staining and a heterotypic cell pair showed α-actinin positive for the CMC and α-actinin negative for the MSC

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