Direct visualization of supercoiled DNA molecules in solution - PubMed (original) (raw)

Direct visualization of supercoiled DNA molecules in solution

M Adrian et al. EMBO J. 1990 Dec.

Abstract

The shape of supercoiled DNA molecules in solution is directly visualized by cryo-electron microscopy of vitrified samples. We observe that: (i) supercoiled DNA molecules in solution adopt an interwound rather than a toroidal form, (ii) the diameter of the interwound superhelix changes from about 12 nm to 4 nm upon addition of magnesium salt to the solution and (iii) the partition of the linking deficit between twist and writhe can be quantitatively determined for individual molecules.

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