Neuropeptide YY(2)R blockade in the central amygdala reduces anxiety-like behavior but not alcohol drinking in alcohol-dependent rats - PubMed (original) (raw)

Neuropeptide YY(2)R blockade in the central amygdala reduces anxiety-like behavior but not alcohol drinking in alcohol-dependent rats

Marsida Kallupi et al. Addict Biol. 2014 Sep.

Abstract

Electrophysiological data suggest a dual role of Y2 receptors (Y2 Rs) as autoreceptors regulating neuropeptide Y release and heteroceptors regulating gamma-aminobutyric acid release in the central amygdala (CeA). Here, we report that neither systemic (JNJ-31020028) nor intra-CeA (BIIE0246) Y2 R antagonism altered operant alcohol responding by alcohol-dependent or non-dependent rats. Conversely, BIIE0246 in the CeA reduced anxiety-like behavior in alcohol-dependent and alcohol-naïve rats. The finding that Y2 R antagonism reduces anxiety-like behavior but not alcohol drinking suggests that these two effects may occur via different functions of the Y2 R (e.g. autoreceptor versus heteroceptor function).

Keywords: Alcohol dependence; Y2 receptor; anxiety; negative reinforcement; self-administration; vapor model.

© 2013 Society for the Study of Addiction.

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Figures

Figure 1

Figure 1

(a) Mean ± SEM lever presses for alcohol by alcohol-dependent and non-dependent rats, and (b) %open-arm time and (c) closed-arm entries in the elevated plus-maze by alcohol-dependent and alcohol-naïve rats following bilateral intra-CeA infusion (30 minutes prior to operant test) of BIIE0246. Rats (n = 14/group) in the self-administration experiment were infused with four doses (0, 5, 50 and 500 ng; 0.5 μL/side) of BIIE0246, and rats (n = 5-6/group) in the elevated plus-maze experiment were infused with one of two doses (0, 50 ng; 0.5 μL/side) of BIIE0246. *P < 0.05 relative to vehicle condition; +P < 0.05 relative to non-dependent controls; #P = 0.07 tendency toward significant difference form naïve controls

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