A resource for cell line authentication, annotation and quality control - PubMed (original) (raw)

. 2015 Apr 16;520(7547):307-11.

doi: 10.1038/nature14397.

Suresh K Selvaraj 1, May M Y Liang-Chu 1, Sahar Aghajani 2, Matthew Busse 2, Jean Yuan 2, Genee Lee 1, Franklin Peale 3, Christiaan Klijn 2, Richard Bourgon 2, Joshua S Kaminker 2, Richard M Neve 1

Affiliations

• PMID: 25877200
• DOI: 10.1038/nature14397

A resource for cell line authentication, annotation and quality control

Mamie Yu et al. Nature. 2015.

Abstract

Cell line misidentification, contamination and poor annotation affect scientific reproducibility. Here we outline simple measures to detect or avoid cross-contamination, present a framework for cell line annotation linked to short tandem repeat and single nucleotide polymorphism profiles, and provide a catalogue of synonymous cell lines. This resource will enable our community to eradicate the use of misidentified lines and generate credible cell-based data.

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