Gene expression elucidates functional impact of polygenic risk for schizophrenia - PubMed (original) (raw)

Figure 3. Neuroanatomical phenotypes upon suppression or overexpression of genes at SCZ risk loci

(a) Head size phenotype after suppression of furin_a (3ng MO) or overexpression of TSNARE1, CNTN4, SNAP91 or CLCN3 (200ng). Representative head size images per treatment condition are shown, quantified area is depicted by the dashed white lines in the control image. (b) Quantification of head size phenotype in each treatment condition as compared to control embryos for furin MO (_N_control = 76, Nfurin MO = 66, P = 5.32 × 10−20), TSNARE1 (N_control = 78, N_TSNARE1 = 64, P = 4.69 × 10−5), CNTN4 (N_control = 66, N_CNTN4 = 75, P = 0.018), SNAP91 (N_control = 114, N_SNAP91 = 106, p = 0.57), CLCN3 (N_control = 114, N_CLCN3 = 100, P = 0.40). (c) Representative images of PH3 staining assessing proliferation phenotypes. Dashed blue lines depict the area included in the quantification of cell counts. (d) Quantification of PH3-labeled cells with respect to each treatment condition for furin MO (_N_control = 19, Nfurin MO = 20, P = 7.56 × 10−17), TSNARE1 (_N_control = 40, NTSNARE1 = 40, P = 0.018), CNTN4 (_N_control = 39, NCNTN4 = 38, P = 0.0032), SNAP91 (N_control = 40, N_SNAP91 = 40, P = 0.25), CLCN3 (_N_control = 40, NCLCN3 = 40, P = 0.07). (e) Representative images of TUNEL staining per condition marking cells undergoing apoptosis. Area quantified is depicted within the dashed blue lines. (f) Cell counts of apoptotic cells in each treatment condition as compared to controls for furin MO (_N_control = 33, Nfurin MO = 39, P = 1.10 × 10−10), TSNARE1 (Ncontrol = 33, NTSNARE1 = 38, P = 9.44 × 10−6), CNTN4 (_N_control = 33, NCNTN4 = 35, P = 1.98 × 10−8). Error bars are s.e., * P < 0.05, ** P < 0.005, *** P < 0.0005; MO - morpholino. Scale bar = 100 µm. In all cases, t- tests were used to generate P values.