Assessment of radicular dentin permeability after irradiation with CO2 laser and endodontic irrigation treatments with thermal imaging - PubMed (original) (raw)

. 2017 Jan 28:10044:100440K.

doi: 10.1117/12.2256735. Epub 2017 Feb 8.

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Assessment of radicular dentin permeability after irradiation with CO2 laser and endodontic irrigation treatments with thermal imaging

Heajin Cho et al. Proc SPIE Int Soc Opt Eng. 2017.

Abstract

Previous studies have demonstrated that the permeability changes due to the surface modification of dentin can be quantified via thermal imaging during dehydration. The CO2 laser has been shown to remove the smear layer and disinfect root canals. Moreover, thermal modification via CO2 laser irradiation can be used to convert dentin into a highly mineralized enamel-like mineral. The purpose of this study is to evaluate the radicular dentin surface modification after CO2 laser irradiation by measuring the permeability with thermal imaging. Human molar specimens (n=12) were sectioned into 4 axial walls of the pulp chamber and treated with either 10% NaClO for 1 minute, 5% EDTA for 1 minute, CO2 laser or none. The CO2 laser was operated at 9.4 μm with a pulse duration of 26 μs, pulse repetition rate of 300 Hz and a fluence of 13 J/cm2. The samples were dehydrated using an air spray for 60 seconds and imaged using a thermal camera. The resulting surface morphological changes were assessed using 3D digital microscopy. The images from digital microscopy confirmed melting of the mineral phase of dentin. The area enclosed by the time-temperature curve during dehydration, ΔQ, measured with thermal imaging increased significantly with treatments with EDTA and the CO2 laser (P<0.05). These results indicate that the surface modification due to CO2 laser treatment increases permeability of radicular dentin.

Keywords: CO2 laser; Root canal therapy; dentin; permeability; thermal Imaging.

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Figures

Fig. 1

Fig. 1

A human molar was cut (blue lines) into 4 sections exposing the axial wall of the pulp chamber.

Fig. 2

Fig. 2

Dehydration experimental setup. CAM: thermal camera, A: compressed air nozzle, S: sample, and WB: water bath

Fig. 3

Fig. 3

DCDM images with magnifications of 150x and 1000x of (A) sound radicular dentin surface, (B) radicular dentin surface treated with 10% NaClO for 1 minute, (C) radicular dentin surface treated with 5% EDTA for 1 minute, and (D) radicular dentin surface treated with the laser.

Fig. 4

Fig. 4

ΔQ measurements ± S.D. after treatments with different endodontic irrigation solutions and the CO2 laser. Bars not sharing any common colors are significantly different, P < 0.05.

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