Contractile effect of short-chain fatty acids on the isolated colon of the rat - PubMed (original) (raw)

Contractile effect of short-chain fatty acids on the isolated colon of the rat

T Yajima. J Physiol. 1985 Nov.

Abstract

The contractile effect of short-chain fatty acids on proximal, middle and distal segments of the rat colon was studied in vitro. A single contraction of the longitudinal muscle of the everted preparation of the middle and distal but not the proximal colon was induced by mucosal application of propionate, butyrate or valerate. Sigmoid dose-responses were observed between contraction and log dose of propionate, butyrate and valerate. The threshold concentration of short-chain fatty acids was between 0.02 and 0.04 mM. A maximal contraction was induced with 0.1 mM-propionate, butyrate and valerate. While acetate (up to 10 mM) and lactate (up to 30 mM) had no contractile effect at all. Serosal application of short-chain fatty acids was without effect, while the contractile response with up to 10 mM-propionate was abolished in both the middle and distal colon by scraping away the mucosa. Cumulative addition of short-chain fatty acids to the organ bath (without wash-out of the first dose) caused adaptation of the contractile response; thus, the effect of propionate (1 mM) was abolished by prior addition of acetate (10 mM) or lactate (30 mM) or propionate (1 mM) or butyrate (1 mM) or valerate (1 mM). The contractile effect of propionate was also inhibited by atropine (1 microM), procaine (0.4 mM) and tetrodotoxin (3 microM); was unaffected by hexamethonium (0.1 mM) and enhanced by eserine (10 nM). The results suggest that short-chain fatty acids, which are normal constituents of the colon, have the ability to stimulate colonic contractions, probably via an enteric reflex involving local sensory and cholinergic nerves.

PubMed Disclaimer

Similar articles

• Luminal propionate-induced secretory response in the rat distal colon in vitro.
  Yajima T. Yajima T. J Physiol. 1988 Sep;403:559-75. doi: 10.1113/jphysiol.1988.sp017264. J Physiol. 1988. PMID: 2473196 Free PMC article.
• Effect of sodium propionate on the contractile response of the rat ileum in situ.
  Yajima T. Yajima T. Jpn J Pharmacol. 1984 Jul;35(3):265-71. doi: 10.1254/jjp.35.265. Jpn J Pharmacol. 1984. PMID: 6482090
• Neural and non-neural mediation of propionate-induced contractile responses in the rat distal colon.
  Mitsui R, Ono S, Karaki S, Kuwahara A. Mitsui R, et al. Neurogastroenterol Motil. 2005 Aug;17(4):585-94. doi: 10.1111/j.1365-2982.2005.00669.x. Neurogastroenterol Motil. 2005. PMID: 16078948
• Roles of short-chain fatty acids receptors, GPR41 and GPR43 on colonic functions.
  Tazoe H, Otomo Y, Kaji I, Tanaka R, Karaki SI, Kuwahara A. Tazoe H, et al. J Physiol Pharmacol. 2008 Aug;59 Suppl 2:251-62. J Physiol Pharmacol. 2008. PMID: 18812643 Review.
• Effects of short-chain fatty acids on gastrointestinal motility.
  Cherbut C, Aubé AC, Blottière HM, Galmiche JP. Cherbut C, et al. Scand J Gastroenterol Suppl. 1997;222:58-61. doi: 10.1080/00365521.1997.11720720. Scand J Gastroenterol Suppl. 1997. PMID: 9145449 Review.

Cited by

• Non-neuronal, but atropine-sensitive ileal contractile responses to short-chain fatty acids: age-dependent desensitization and restoration under inflammatory conditions in mice.
  Yajima M, Kimura S, Karaki S, Nio-Kobayashi J, Tsuruta T, Kuwahara A, Yajima T, Iwanaga T. Yajima M, et al. Physiol Rep. 2016 Apr;4(7):e12759. doi: 10.14814/phy2.12759. Physiol Rep. 2016. PMID: 27053293 Free PMC article.
• The structural alteration of gut microbiota in low-birth-weight mice undergoing accelerated postnatal growth.
  Wang J, Tang H, Wang X, Zhang X, Zhang C, Zhang M, Zhao Y, Zhao L, Shen J. Wang J, et al. Sci Rep. 2016 Jun 9;6:27780. doi: 10.1038/srep27780. Sci Rep. 2016. PMID: 27277748 Free PMC article.
• Expression of the short-chain fatty acid receptor, GPR43, in the human colon.
  Karaki S, Tazoe H, Hayashi H, Kashiwabara H, Tooyama K, Suzuki Y, Kuwahara A. Karaki S, et al. J Mol Histol. 2008 Apr;39(2):135-42. doi: 10.1007/s10735-007-9145-y. Epub 2007 Sep 27. J Mol Histol. 2008. PMID: 17899402
• Effects of butyric acid and analogues on amylase release from pancreatic segments of sheep and goats.
  Katoh K, Yajima T. Katoh K, et al. Pflugers Arch. 1989 Jan;413(3):256-60. doi: 10.1007/BF00583538. Pflugers Arch. 1989. PMID: 2470024
• A Novel Antagonist Peptide Reveals a Physiological Role of Insulin-Like Peptide 5 in Control of Colorectal Function.
  Pustovit RV, Zhang X, Liew JJ, Praveen P, Liu M, Koo A, Oparija-Rogenmozere L, Ou Q, Kocan M, Nie S, Bathgate RA, Furness JB, Hossain MA. Pustovit RV, et al. ACS Pharmacol Transl Sci. 2021 Aug 30;4(5):1665-1674. doi: 10.1021/acsptsci.1c00171. eCollection 2021 Oct 8. ACS Pharmacol Transl Sci. 2021. PMID: 34661082 Free PMC article.

References

1. 1. J Physiol. 1984 Jan;346:379-93 - PubMed
2. 1. Jpn J Physiol. 1959 Mar 25;9(1):49-55 - PubMed
3. 1. Br J Nutr. 1980 Jan;43(1):155-62 - PubMed
4. 1. Life Sci. 1977 Jul 1;21(1):59-62 - PubMed
5. 1. J Physiol. 1900 Dec 31;26(1-2):107-18 - PubMed

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • PubMed Central
  • Wiley