Glucose, glutamine and ketone body utilisation by resting and concanavalin A activated rat splenic lymphocytes - PubMed (original) (raw)
Glucose, glutamine and ketone body utilisation by resting and concanavalin A activated rat splenic lymphocytes
A M O'Rourke et al. Biochim Biophys Acta. 1989.
Abstract
The utilisation of glucose, glutamine, acetoacetate and D-3-hydroxybutyrate were investigated over 72 h of incubation of rat splenic lymphocytes, with and without concanavalin A. Lymphocytes consumed both ketone bodies; acetoacetate was consumed preferentially. The ketone bodies reduced glucose consumption by 30-50%, but had little effect on lactate production. Glutamine uptake was concentration dependent up to 4 mM, and consumption was increased in the presence of concanavalin. Glutamine stimulated glucose consumption and lactate production in both resting and activated cells. Complete oxidation contributed 65% of glucose-derived ATP, but less than 40% of glutamine-derived ATP. Glutamine metabolism makes only a minor contribution to lymphocyte ATP generation.
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