CHANGES IN MICROTUBULES OF CILIA AND FLAGELLA FOLLOWING NEGATIVE STAINING WITH PHOSPHOTUNGSTIC ACID - PubMed (original) (raw)
. 1970 Oct;139(2):265-276.
doi: 10.2307/1540082.
- PMID: 29332463
- DOI: 10.2307/1540082
CHANGES IN MICROTUBULES OF CILIA AND FLAGELLA FOLLOWING NEGATIVE STAINING WITH PHOSPHOTUNGSTIC ACID
Catherine Henley. Biol Bull. 1970 Oct.
Abstract
1. Variability in the macerating action of 1% aqueous phosphotungstic acid, pH 6.8, is exemplified in microtubules of flatworm and rhynchocoel cilia, from one grid square to another of the same preparation and within a single grid square. The central singlets appear to be the most susceptible and are often completely absent, even in cases where the binding matrix is still present around the doublets. Maceration usually, but not always, begins at the distal tip of a cilium and proceeds towards the basal plate; it is evident along the lengths of doublets as partial or complete loss of one subtubule, as breaks and bends, and as fraying into the component protofibrils, with disappearance of the white line marking the wall between the subtubules. 2. Cortical singlet microtubules of a spermatozoon were thrown into a helical configuration by the action of PTA, while the flagellar microtubules of the same spermatozoon were unaffected. 3. After treatment with 1% PTA at pH 8.3, the central singlets of cilia (but not the doublets) were semi-fused along most of their lengths, and had a beaded appearance; there were breaks in the doublets but otherwise they appeared to be unaffected. 4. Ciliary rootlets were rarely seen, but when present had a clear 790 Å major repeating pattern and a longitudinal fibrous substructure.
Similar articles
- Asymmetrical microtubule capping structures in frog palate cilia.
LeCluyse EL, Dentler WL. LeCluyse EL, et al. J Ultrastruct Res. 1984 Jan;86(1):75-85. doi: 10.1016/s0022-5320(84)90097-2. J Ultrastruct Res. 1984. PMID: 6737561 - Bizarre flagellum of thrips spermatozoa (Thysanoptera, Insecta).
Dallai R, Afzelius BA, Lanzavecchia S, Bellon PL. Dallai R, et al. J Morphol. 1991 Sep;209(3):343-347. doi: 10.1002/jmor.1052090309. J Morphol. 1991. PMID: 29865540 - Observations on the solitary cilium of rabbit oviductal epithelium: its motility and ultrastructure.
Odor DL, Blandau RJ. Odor DL, et al. Am J Anat. 1985 Dec;174(4):437-53. doi: 10.1002/aja.1001740407. Am J Anat. 1985. PMID: 3909797 - Testing the geometric clutch hypothesis.
Lindemann CB. Lindemann CB. Biol Cell. 2004 Dec;96(9):681-90. doi: 10.1016/j.biolcel.2004.08.001. Biol Cell. 2004. PMID: 15567522 Review. - The ciliary cytoskeleton.
Pedersen LB, Schrøder JM, Satir P, Christensen ST. Pedersen LB, et al. Compr Physiol. 2012 Jan;2(1):779-803. doi: 10.1002/cphy.c110043. Compr Physiol. 2012. PMID: 23728985 Review.
LinkOut - more resources
Full Text Sources