First report of Paragonimus skrjabini miyazakii metacercariae in Geothelphusa dehaani (Sawagani) occurring in Iwate Prefecture, Japan - PubMed (original) (raw)

First report of Paragonimus skrjabini miyazakii metacercariae in Geothelphusa dehaani (Sawagani) occurring in Iwate Prefecture, Japan

Yuma Ohari et al. J Vet Med Sci. 2019.

Abstract

Paragonimiasis is an important food-borne zoonosis caused by Paragonimus flukes and is endemic to western Japan. However, there have been few epidemiological studies in the Tohoku district of northeastern Japan. In this study, Paragonimus metacercariae (mc) was detected in Geothelphusa dehaani (Japanese freshwater crab or Sawagani) in Iwate Prefecture. Out of the 207 Sawagani collected from 35 localities, 12 individuals from six localities were infected with Paragonimus mc. The mc were identified as P. skrjabini miyazakii based on the sequences of nuclear ribosomal internal transcribed spacer 2 and mitochondrial cytochrome c oxidase I. This is the first report of P. s. miyazakii mc infection in Sawagani in Iwate Prefecture.

Keywords: Geothelphusa dehaani; Iwate Prefecture; Paragonimus skrjabini miyazakii; cox1; nuclear ribosomal internal transcribed spacer 2.

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Figures

Fig. 1.

Fig. 1.

Map of Iwate Prefecture showing the sampling localities. The black crosses and gray circles show localities from where _Paragonimus skrjabini miyazakii_metacercariae infected and non-infected Sawagani, respectively, were collected. The numbers indicate the locality numbers shown in Table 1.

Fig. 2.

Fig. 2.

A maximum likelihood tree constructed based on cox1 seuences of_Paragonimus_ species. The numbersnear the nodes indicate bootstrap values (>90%). The scale bar indicates the number of substitutions per sequence position.

References

    1. Blair D., Agatsuma T., Watanobe T., Okamoto M., Ito A.1997. Geographical genetic structure within the human lung fluke, Paragonimus westermani, detected from DNA sequences. Parasitology 115: 411–417. doi: 10.1017/S0031182097001534 - DOI - PubMed
    1. Blair D., Xu Z. B., Agatsuma T.1999. Paragonimiasis and the genus Paragonimus. Adv. Parasitol. 42: 113–222. doi: 10.1016/S0065-308X(08)60149-9 - DOI - PubMed
    1. Blair D., Chang Z., Chen M., Cui A., Wu B., Agatsuma T., Iwagami M., Corlis D., Fu C., Zhan X.2005. Paragonimus skrjabini Chen, 1959 (Digenea: Paragonimidae) and related species in eastern Asia: a combined molecular and morphological approach to identification and taxonomy. Syst. Parasitol. 60: 1–21. doi: 10.1007/s11230-004-1378-5 - DOI - PubMed
    1. Bowles J., Blair D., McManus D. P.1995. A molecular phylogeny of the human schistosomes. Mol. Phylogenet. Evol. 4: 103–109. doi: 10.1006/mpev.1995.1011 - DOI - PubMed
    1. Hasegawa M., Kishino H., Yano T.1985. Dating of the human-ape splitting by a molecular clock of mitochondrial DNA. J. Mol. Evol. 22: 160–174. doi: 10.1007/BF02101694 - DOI - PubMed

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