Identification and quantitation of platelet-associated fibronectin antigen - PubMed (original) (raw)

Identification and quantitation of platelet-associated fibronectin antigen

E F Plow et al. J Clin Invest. 1979 Mar.

Abstract

Platelet-associated fibronectin antigen has been identified by radioimmunoassay and immunofluorescent techniques. In radioimmunoassay, platelet fibronectin was immunochemically indistinguishable from plasma fibronectin. Platelet and plasma fibronectin were bound and eluted from gelatin-sepharose under similar conditions. The level of platelet fibronectin in detergent extracts of washed platelets from 12 healthy adults was 2.85 +/- 1.24 microgram/10(9) platelets. Immunofluorescence with F(ab')2 fragments of immunochemically purified antifibronectin showed that all platelets stained with a discrete punctate pattern. The identification of platelet fibronectin antigen raises the possibility that this protein may participate in platelet-platelet or platelet-surface interactions.

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References

    1. Nature. 1978 Sep 21;275(5677):179-84 -PubMed
    1. Biochim Biophys Acta. 1978 Sep 18;516(1):1-25 -PubMed
    1. Thromb Haemost. 1977 Dec 15;38(4):924-38 -PubMed
    1. Proc Natl Acad Sci U S A. 1977 Sep;74(9):4033-6 -PubMed
    1. J Clin Invest. 1977 Nov;60(5):999-1007 -PubMed

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