Rooting for success: Evolutionary enhancement of Bacillus for superior plant colonization - PubMed (original) (raw)

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Rooting for success: Evolutionary enhancement of Bacillus for superior plant colonization

Vincent Charron-Lamoureux et al. Microb Biotechnol. 2024 Sep.

Abstract

Many strains from the Bacillus subtilis species complex exert strong plant growth-promoting activities. However, their efficacy in relevant conditions is variable, due in part to their inability to establish a strong interaction with roots in stressful environmental conditions. Adaptative laboratory evolution (ALE) is a powerful tool to generate novel strains with traits of interest. Many Bacillus evolved isolates, stemming from ALE performed with plants, possess a stronger root colonization capacity. An in-depth analysis of these isolates also allowed the identification of key features influencing the interaction with plant roots. However, many variables can influence the outcome of these assays, and thus, caution should be taken when designing ALE destined to generate better root colonizers.

© 2024 The Author(s). Microbial Biotechnology published by John Wiley & Sons Ltd.

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Conflict of interest statement

The authors declare no conflict of interests.

Figures

FIGURE 1

FIGURE 1

The many parameters of adaptative laboratory experiments. The various parameters chosen in an ALE protocol (in bold green) can influence the outcome of the evolution, leading to adaptations with various fitness to the natural rhizosphere. Subsequent steps should include validation of the colonization efficacy and whole‐genome sequencing to determine the driver of adaptations.

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