Brain morphological analysis in mice with hyperactivation of the hedgehog signaling pathway - PubMed (original) (raw)
doi: 10.3389/fnins.2024.1449673. eCollection 2024.
Hideki Uchikawa 1 2, Nobuhiro Nitta 3 4, Tomozumi Takatani 1, Shingo Matsuda 5 6 7, Alpen Ortug 8 9, Emi Takahashi 8 9, Daisuke Sawada 1, Eiji Shimizu 5, Katsunori Fujii 1 10, Ichio Aoki 3, Hiromichi Hamada 1
Affiliations
- PMID: 39290714
- PMCID: PMC11405378
- DOI: 10.3389/fnins.2024.1449673
Brain morphological analysis in mice with hyperactivation of the hedgehog signaling pathway
Tadashi Shiohama et al. Front Neurosci. 2024.
Abstract
Hedgehog signaling is a highly conserved pathway that plays pivotal roles in morphogenesis, tumorigenesis, osteogenesis, and wound healing. Previous investigations in patients with Gorlin syndrome found low harm avoidance traits, and increased volumes in the cerebrum, cerebellum, and cerebral ventricles, suggesting the association between brain morphology and the constitutive hyperactivation of hedgehog signaling, while the changes of regional brain volumes in upregulated hedgehog signaling pathway remains unclear so far. Herein, we investigated comprehensive brain regional volumes using quantitative structural brain MRI, and identified increased volumes of amygdala, striatum, and pallidum on the global segmentation, and increased volumes of the lateral and medial parts of the central nucleus of the amygdala on the detail segmentation in Ptch heterozygous deletion mice. Our data may enhance comprehension of the association between brain morphogenic changes and hyperactivity in hedgehog signaling.
Keywords: Gorlin syndrome; Ptch; brain magnetic resonance imaging; hedgehog signaling; laterality.
Copyright © 2024 Shiohama, Uchikawa, Nitta, Takatani, Matsuda, Ortug, Takahashi, Sawada, Shimizu, Fujii, Aoki and Hamada.
Conflict of interest statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Figures
Figure 1
Brain morphology analysis using MRI. T2 weighted images (A), atlas registration (B), quantification of anatomical regions using volume-based morphometry (C), and laterality index (D) in wild type (WT) and Ptch heterozygous deletion (PTCH+/−) mice. * indicates significantly difference (p < 0.05).
References
Grants and funding
The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. This study was funded by the Japan Society for the Promotion of Science (JSPS) KAKENHI JP17K10048 (UH), JP17K16241 (TS), JP20K08252 (TS), JP23K07326 (KF), JP21K07694 (TS), and JP24K02392 (TS). MRI devices were partly supported by Project for promoting public utilization of advanced research infrastructure (JPMXS0450400422, JST/MEXT) and COI-NEXT Program (JPMJPF2202, JST).
LinkOut - more resources
Full Text Sources