Isolation of a blaNDM-1-positive strain in Israel predating the earliest observations from India - PubMed (original) (raw)

Isolation of a _bla_NDM-1-positive strain in Israel predating the earliest observations from India

Frédéric Grenier et al. Microbiol Spectr. 2024.

Abstract

_bla_NDM, the most prevalent carbapenemase among carbapenem-resistant Enterobacteriaceae, is thought to have emerged in India, as its initial detection in 2008 was linked to this country, and subsequent retrospective surveys had so far established the earliest _bla_NDM-positive strains to be isolated in India in 2005. Molecular dating and analyses suggest bla_NDM emerged within Acinetobacter species decades prior to 2005 on a Tn_125 transposon. Despite early reports of elevated rates of carbapenem-resistant Acinetobacter species in Israel starting in the 1990s, limited molecular data are available from this location. We searched for _bla_NDM among Acinetobacter species isolated in Israel between 2001 and 2006. One A. junii strain, Ajun-H1-3, isolated in January 2004, carried _bla_NDM-1 within a Tn_125_-like transposon on a 49-kb plasmid, pNDM-Ajun-H1-3, making Ajun-H1-3 the earliest NDM-positive isolate observed to date. The pNDM-Ajun-H1-3 plasmid matched numerous BJ01-like NDM-positive plasmids identified from 2005 onward in Acinetobacter species as well as Enterobacterales. These results indicate the need for further retrospective work on global strain archives to shed light on the conditions favoring the emergence as well as subsequent evolution and spread of _bla_NDM.

Importance: This study presents the earliest observation of _bla_NDM-1, isolated in a geographical region distant from where it is believed to have originated. In doing so, this study provides novel insights into the emergence and spread of _bla_NDM, the most prevalent carbapenemase among carbapenem-resistant Enterobacteriaceae, and its associated mobile genetic elements. It also sheds light on the conditions that foster the evolution of antimicrobial resistance, one of the greatest public health challenges we face.

Keywords: Acinetobacter junii; Antimicrobial resistance; BJ01-like plasmid; Tn125 transposon; blaNDM.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Fig 1

Fig 1

Comparison and alignment of certain BJ01-like plasmids in Acinetobacter spp. and Enterobacterales. Panel 1 (A) Genetic structure of the Tn_125_-like transposon carrying bla_NDM-1 within pNDM-Ajun-H1-3, containing an additional aph(3’)-VIa gene and additional IS_Aba125 insertion sequence compared to other Tn_125_ transposons. Panel 1 (B) The top five plasmids are the only BJ01-like plasmids deposited in GenBank lacking _bla_NDM (presented in chronological order from top to bottom). Below these, some of the _bla_NDM-containing BJ01-like plasmids are also present in chronological order from top to bottom, starting with the earliest known observation of _bla_NDM-1 reported as part of this study. A color gradient of the link identity percentage presents genetic sequence similarities. Alignments demonstrate dissemination of highly conserved plasmids: pNDM-Ajun-H1-3 is 99.99% similar over 97% of pNDM-40–1’s sequence and 99.98% similar over 97% of pNDM-BJ01’s sequence. The species identification, country, year of isolation, and GenBank accession number are listed underneath the plasmid name. Although data for pNDM-JVAP01 were already available (11), the assembly presented here is derived from our own sequencing.

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