Cooperative binding of lambda repressors to sites separated by integral turns of the DNA helix - PubMed (original) (raw)
Cooperative binding of lambda repressors to sites separated by integral turns of the DNA helix
A Hochschild et al. Cell. 1986.
Abstract
Lambda repressors bind cooperatively to adjacent pairs of operator sites. Here we show that repressors bind cooperatively to pairs of operator sites whose centers have been separated by five or six turns of the helix. No cooperativity is observed when the centers of these sites are on opposite sides of the DNA helix. Cooperativity depends upon the same part of the protein (the carboxyl domain) that mediates cooperativity when the sites are adjacent. As the repressors bind, the DNA between the sites becomes alternately sensitive and resistant to DNAase I cleavage at half turn intervals. We suggest that when repressors bind cooperatively to separated sites, the DNA forms a loop, thus allowing the two repressors to touch.
Similar articles
- DNA loops induced by cooperative binding of lambda repressor.
Griffith J, Hochschild A, Ptashne M. Griffith J, et al. Nature. 1986 Aug 21-27;322(6081):750-2. doi: 10.1038/322750a0. Nature. 1986. PMID: 3748156 - A variant of lambda repressor with an altered pattern of cooperative binding to DNA sites.
Astromoff A, Ptashne M. Astromoff A, et al. Proc Natl Acad Sci U S A. 1995 Aug 29;92(18):8110-4. doi: 10.1073/pnas.92.18.8110. Proc Natl Acad Sci U S A. 1995. PMID: 7667253 Free PMC article. - Recognition of a DNA operator by the repressor of phage 434: a view at high resolution.
Aggarwal AK, Rodgers DW, Drottar M, Ptashne M, Harrison SC. Aggarwal AK, et al. Science. 1988 Nov 11;242(4880):899-907. doi: 10.1126/science.3187531. Science. 1988. PMID: 3187531 - Comparison of the structures of operator DNA free and in complex with lambda repressor.
Baleja JD, Sykes BD. Baleja JD, et al. Biochem Cell Biol. 1991 Feb-Mar;69(2-3):202-5. doi: 10.1139/o91-030. Biochem Cell Biol. 1991. PMID: 2031722 Review. - Protein-DNA recognition.
Pabo CO, Sauer RT. Pabo CO, et al. Annu Rev Biochem. 1984;53:293-321. doi: 10.1146/annurev.bi.53.070184.001453. Annu Rev Biochem. 1984. PMID: 6236744 Review.
Cited by
- Domains of the rat rDNA promoter must be aligned stereospecifically.
Xie WQ, Rothblum LI. Xie WQ, et al. Mol Cell Biol. 1992 Mar;12(3):1266-75. doi: 10.1128/mcb.12.3.1266-1275.1992. Mol Cell Biol. 1992. PMID: 1545808 Free PMC article. - The quaternary structure of Tet repressors bound to the Tn10-encoded tet gene control region determined by neutron solution scattering.
Lederer H, Tovar K, Baer G, May RP, Hillen W, Heumann H. Lederer H, et al. EMBO J. 1989 Apr;8(4):1257-63. doi: 10.1002/j.1460-2075.1989.tb03499.x. EMBO J. 1989. PMID: 2545442 Free PMC article. - Stereospecific relationships between elements in an SV40/adenovirus-2 heterologous promoter.
Lennard AC, Matthes HW, Egly JM, Chambon P. Lennard AC, et al. Nucleic Acids Res. 1989 Sep 12;17(17):6903-14. doi: 10.1093/nar/17.17.6903. Nucleic Acids Res. 1989. PMID: 2550902 Free PMC article. - Regulation of the Bacillus subtilis W23 xylose utilization operon: interaction of the Xyl repressor with the xyl operator and the inducer xylose.
Gärtner D, Degenkolb J, Ripperger JA, Allmansberger R, Hillen W. Gärtner D, et al. Mol Gen Genet. 1992 Apr;232(3):415-22. doi: 10.1007/BF00266245. Mol Gen Genet. 1992. PMID: 1588910 - Alternative DNA-protein interactions in variable-length internucleosomal regions associated with Drosophila Adh distal promoter expression.
Ewel A, Jackson JR, Benyajati C. Ewel A, et al. Nucleic Acids Res. 1990 Apr 11;18(7):1771-81. doi: 10.1093/nar/18.7.1771. Nucleic Acids Res. 1990. PMID: 2159621 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials