In vivo evidence that bacteria in urinary tract infection grow under iron-restricted conditions - PubMed (original) (raw)

In vivo evidence that bacteria in urinary tract infection grow under iron-restricted conditions

G H Shand et al. Infect Immun. 1985 Apr.

Abstract

The outer membrane protein composition of bacteria isolated directly and without subculturing from the urine of two patients with urinary tract infections was investigated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The results indicated that the bacteria grew under iron-restricted conditions, as revealed by the expression of several high-molecular-weight outer membrane proteins which could also be observed when the same isolates were grown under iron-depleted conditions in laboratory media. The antigenicity of outer membrane components of the bacteria isolated was studied by immunoblotting with serum samples from the patients. The results indicated that the sera from the patients contained antibodies against major outer membrane components of the bacteria present in the urine, including the iron-regulated membrane proteins.

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