New benzoyl sesquiterpenoid, aoganolide, produced by Talaromyces sp. KTF-0021 strain (laeA-introduced mutant of FKI-5759 strain) - PubMed (original) (raw)

. 2025 Sep;78(10):586-592.

doi: 10.1038/s41429-025-00851-6. Epub 2025 Jul 25.

Yoshihiro Watanabe # 1 2, Yuta Kikuchi 1 2, Hayama Tsutsumi 1 2, Haruki Azami 1, Hiroki Kojima 1 2, Sho Kato 1 2, Akihiro Sugawara 1 2, Yuki Inahashi 1 2, Kenichi Nonaka 1 2, Rei Hokari 1 2, Aki Ishiyama 1 2, Yasuko Araki 3, Kona Yamashita 3, Tadashi Takahashi 3, Kotaro Ito 3, Yukihiro Asami 1 2, Masato Iwatsuki 4 5

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New benzoyl sesquiterpenoid, aoganolide, produced by Talaromyces sp. KTF-0021 strain (laeA-introduced mutant of FKI-5759 strain)

Karin Hamada et al. J Antibiot (Tokyo). 2025 Sep.

Abstract

To discover novel natural compounds by awakening unexplored or silent BGCs in fungi, our research group has explored the cultured broths of the laeA-introduced mutant fungal strains. In this study, we report the isolation of a new benzoyl sesquiterpenoid, aoganolide (4), as well as three known compounds, decarboxyaltenusin (1), altenusin (2), and penipyranicin B (3), from the cultured broth of Talaromyces sp. KTF-0021, which was a laeA-introduced mutant of FKI-5759 strain. The planar structure of 4 was elucidated by spectroscopic analysis and the absolute configuration of 4 was determined by the calculated ECD spectral method. Among them, 4 showed antimalarial activity with IC50 values of 4.37 and 6.46 µg ml-1 against Plasmodium falciparum FCR3 and K1 strains, respectively. The laeA-introduced mutant strain produced 2, 3, and 4 with higher productivity (47, 623, and 38 mg l-1) than the wild-type strain (1.4, 0.8, and 7.7 mg l-1), respectively, suggesting this method is useful to expand the chemical diversity of natural products.

© 2025. The Author(s), under exclusive licence to the Japan Antibiotics Research Association.

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Conflict of interest statement

Compliance with ethical standards. Conflict of interest: The authors declare no competing interests.

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