Esterification of fatty acids at room temperature by chloroform-methanolic HCl-cupric acetate - PubMed (original) (raw)
. 1973 Sep;14(5):599-601.
- PMID: 4729977
Free article
Esterification of fatty acids at room temperature by chloroform-methanolic HCl-cupric acetate
M Hoshi et al. J Lipid Res. 1973 Sep.
Free article
Abstract
A new procedure for the preparation of methyl esters from free fatty acids under mild conditions was investigated. Free fatty acids are dissolved in a mixture of chloroform-methanolic HCl-cupric acetate and kept at room temperature for 30 min for complete esterification. The method is suitable for esterification of long-chain acids, such as 18:0, and for very long chain acids, such as 24:0. Fatty acids from brain glycerophosphatides, which included a high concentration of polyenes such as 20:4 (n - 6), 22:4 (n - 6), and 22:6 (n - 3), were also esterified by the same procedure, and neither artifact formation nor loss of unsaturated acids was observed.
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