Effect of trypsin and ribonuclease on the immunogenic activity of ribosomes and ribonucleic acid isolated from Mycobacterium tuberculosis - PubMed (original) (raw)
Effect of trypsin and ribonuclease on the immunogenic activity of ribosomes and ribonucleic acid isolated from Mycobacterium tuberculosis
A S Youmans et al. J Bacteriol. 1966 Jun.
Abstract
Youmans, Anne S. (Northwestern University Medical School, Chicago, Ill.), and Guy P. Youmans. Effect of trypsin and ribonuclease on the immunogenic activity of ribosomes and ribonucleic acid isolated from Myobacterium tuberculosis. J. Bacteriol. 91:2146-2154. 1966.-The ribosomal fraction of the attenuated strain, H37Ra, of Mycobacterium tuberculosis was treated with trypsin alone, ethylenediaminetetraacetic acid (EDTA) alone, EDTA and pancreatic ribonuclease, or with trypsin and ribonuclease. After each of these treatments, the ribosomal fractions were injected intraperitoneally into male CF-1 mice to test their capacity to produce an immune response to infection with virulent tubercle bacilli, strain H37Rv. Removal of protein with trypsin left the immunogenicity unchanged; EDTA alone reduced immunogenicity in the smaller vaccinating doses; EDTA plus ribonuclease reduced the immunogenicity by approximately 50% in the highest (1.0 mg) vaccinating dose; ribonuclease alone, after treatment with trypsin, reduced immunogenicity also approximately 50%. A crude mycobacterial ribonucleic acid (RNA) was prepared by extraction of the ribosomal fraction with alcohol. This RNA preparation was as effective in producing an immune response as the ribosomal fraction from which it was prepared, unless the RNA was partially or completely degraded during the preparation. The effect of ribonuclease on the immunogenicity of the RNA was similar to that obtained with the ribosomal fractions, except that ribonuclease completely destroyed the immunogenicity of a partially degraded RNA. RNA appears to be an essential part of an immunizing substance in attenuated tubercle bacilli, which produces a high degree of immunity in mice; 50 mug (dry weight) will protect approximately 80% of the mice, and as little as 0.5 mug will protect approximately 30% of the mice. Mycobacterial RNA not incorporated in Freund's incomplete adjuvant was nonimmunogenic. Yeast RNA incorporated in Freund's incomplete adjuvant was not immunogenic.
Similar articles
- Preparation of highly immunogenic ribosomal fractions of Mycobacterium tuberculosis by use of sodium dodecyl sulfate.
Youmans AS, Youmans GP. Youmans AS, et al. J Bacteriol. 1966 Jun;91(6):2139-45. doi: 10.1128/jb.91.6.2139-2145.1966. J Bacteriol. 1966. PMID: 4957609 Free PMC article. - IMMUNOGENIC ACTIVITY OF A RIBOSOMAL FRACTION OBTAINED FROM MYCOBACTERIUM TUBERCULOSIS.
YOUMANS AS, YOUMANS GP. YOUMANS AS, et al. J Bacteriol. 1965 May;89(5):1291-8. doi: 10.1128/jb.89.5.1291-1298.1965. J Bacteriol. 1965. PMID: 14293000 Free PMC article. - NATURE OF THE LABILE IMMUNOGENIC SUBSTANCE IN THE PARTICULATE FRACTION ISOLATED FROM MYCOBACTERIUM TUBERCULOSIS.
YOUMANS AS, YOUMANS GP. YOUMANS AS, et al. J Bacteriol. 1964 Oct;88(4):1030-7. doi: 10.1128/jb.88.4.1030-1037.1964. J Bacteriol. 1964. PMID: 14219015 Free PMC article. - EFFECT OF MITOCHONDRIAL STABILIZERS ON THE IMMUNOGENICITY OF THE PARTICULATE FRACTION ISOLATED FROM MYCOBACTERIUM TUBERCULOSIS.
YOUMANS AS, YOUMANS GP. YOUMANS AS, et al. J Bacteriol. 1964 Jun;87(6):1346-54. doi: 10.1128/jb.87.6.1346-1354.1964. J Bacteriol. 1964. PMID: 14188712 Free PMC article. - Factors affecting immunogenic activity of mycobacterial ribosomal and ribonucleic acid preparations.
Youmans AS, Youmans GP. Youmans AS, et al. J Bacteriol. 1969 Jul;99(1):42-50. doi: 10.1128/jb.99.1.42-50.1969. J Bacteriol. 1969. PMID: 4979447 Free PMC article.
Cited by
- Identification of Mycobacterial Ribosomal Proteins as Targets for CD4+ T Cells That Enhance Protective Immunity in Tuberculosis.
Kennedy SC, Johnson AJ, Bharrhan S, Lindestam Arlehamn CS, Xu J, Garforth SJ, Chan J, Jacobs WR Jr, Sette A, Almo SC, Porcelli SA. Kennedy SC, et al. Infect Immun. 2018 Aug 22;86(9):e00009-18. doi: 10.1128/IAI.00009-18. Print 2018 Sep. Infect Immun. 2018. PMID: 29891545 Free PMC article. - Identification of Mycobacterial RplJ/L10 and RpsA/S1 Proteins as Novel Targets for CD4+ T Cells.
Johnson AJ, Kennedy SC, Lindestam Arlehamn CS, Goldberg MF, Saini NK, Xu J, Paul S, Hegde SS, Blanchard JS, Chan J, Jacobs WR Jr, Sette A, Porcelli SA. Johnson AJ, et al. Infect Immun. 2017 Mar 23;85(4):e01023-16. doi: 10.1128/IAI.01023-16. Print 2017 Apr. Infect Immun. 2017. PMID: 28115505 Free PMC article. - Immunogenicity of Ribosomal Preparations from Yeast Cells of Histoplasma capsulatum.
Feit C, Tewari RP. Feit C, et al. Infect Immun. 1974 Nov;10(5):1091-7. doi: 10.1128/iai.10.5.1091-1097.1974. Infect Immun. 1974. PMID: 16558095 Free PMC article. - Protection against pneumococcal infection by a ribosomal preparation.
Thompson HC, Snyder IS. Thompson HC, et al. Infect Immun. 1971 Jan;3(1):16-23. doi: 10.1128/iai.3.1.16-23.1971. Infect Immun. 1971. PMID: 16557934 Free PMC article. - Failure of synthetic polynucleotides to affect the immunogenicity of mycobacterial ribonucleic Acid and ribosomal protein preparations.
Youmans AS, Youmans GP. Youmans AS, et al. Infect Immun. 1971 Jan;3(1):149-53. doi: 10.1128/iai.3.1.149-153.1971. Infect Immun. 1971. PMID: 16557931 Free PMC article.
References
- J Bacteriol. 1962 Oct;84:708-15 - PubMed
- J Bacteriol. 1964 Oct;88:1030-7 - PubMed
- Biochem J. 1961 Mar;78:457-72 - PubMed
- J Exp Med. 1958 Oct 1;108(4):493-506 - PubMed
- J Bacteriol. 1964 Feb;87:278-85 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources