The effect of triclabendazole ("Fasinex") on protein synthesis by the liver fluke, Fasciola hepatica - PubMed (original) (raw)
Comparative Study
The effect of triclabendazole ("Fasinex") on protein synthesis by the liver fluke, Fasciola hepatica
A W Stitt et al. Int J Parasitol. 1995 Apr.
Abstract
The effect of the active sulphoxide metabolite of the anthelmintic triclabendazole (TCBZ-SX, 15-50 micrograms ml-1) on the incorporation of radioactively labelled [14C] leucine by adult Fasciola hepatica tissue slices was measured by liquid scintillation counting. In addition, the ability of the microfilament-disrupting drug, cytochalasin B, and the microtubule-disrupting drug, tubulozole-C, to inhibit protein synthesis, was assessed by similar methods and compared with TCBZ-SX. The established protein synthesis inhibitors, cycloheximide and actinomycin D were used as positive controls. All the drugs showed a significant inhibition of protein synthesis, albeit to different extents; however, TCBZ-SX was the most potent, with no significant difference between its effect and that of cycloheximide or actinomycin D. Moreover, the concentration of TCBZ-SX, above 15 micrograms ml-1, had little further influence on incorporation of [14C] leucine. This investigation demonstrates the inhibitory effect of TCBZ-SX, cytochalasin B and tubulozole-C on protein synthesis in F. hepatica and confirms the qualitative observations made in several previous ultrastructural studies.