Distribution of anionic sites on the oviduct ciliary membrane - PubMed (original) (raw)
Distribution of anionic sites on the oviduct ciliary membrane
R G Anderson et al. J Cell Biol. 1977 Feb.
Abstract
Polycationic ferritin (PCF) was used as a visual probe for anionic sites on the oviduct ciliary membrane. The binding of PCF to ciliary membranes was dependent on the concentration of the probe in the incubation media. At low concentrations (0.08-0.16 mg/ml), PCF was bound exclusively to the tip of the cilium whereas at higher concentrations (0.32-0.64 mg/ml), ferritin was located at the tip and at the base around the transition region, with occasional scattered clumps on the remainder of the membrane. The base and tip binding was fount to be associated with special surface modifications of the membrane in these regions. At the tip, PCF was bound to a filamentous glycocalyx termed the ciliary crown. Base binding was associated with a system of five to six 140-A high ridges, each of which encircled the membrane of the transition region. The ridges were equally spaced (approxamately 245 A spacing) along the length of the transition region. Since pretreatment of oviduct with either neuraminidase or protease blocked the binding of the probe, the PCF-binding sites appear to be negatively charged glycoproteins or mucopolysaccharides.
Similar articles
- Polycationic macromolecules inhibit cilia-mediated ovum transport in the rabbit oviduct.
Norwood JT, Hein CE, Halbert SA, Anderson RG. Norwood JT, et al. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4413-6. doi: 10.1073/pnas.75.9.4413. Proc Natl Acad Sci U S A. 1978. PMID: 279926 Free PMC article. - The distribution and mobility of anionic sites on the surface of human placental syncytial trophoblast.
King BF. King BF. Anat Rec. 1981 Jan;199(1):15-22. doi: 10.1002/ar.1091990103. Anat Rec. 1981. PMID: 7224198 - Cationized ferritin binding to anionic surfaces in normal and aminonucleoside nephrotic kidneys.
Andrews PM. Andrews PM. Am J Anat. 1981 Oct;162(2):89-106. doi: 10.1002/aja.1001620202. Am J Anat. 1981. PMID: 7304471 - Chronic effects of nitrogen dioxide on cilia in hamster bronchioles.
Heller RF, Gordon RE. Heller RF, et al. Exp Lung Res. 1986;10(2):137-52. doi: 10.3109/01902148609061489. Exp Lung Res. 1986. PMID: 3956447 - Characterization of free surface microprojections on the kidney glomerular epithelium.
Andrews PM. Andrews PM. Prog Clin Biol Res. 1981;59B:21-35. Prog Clin Biol Res. 1981. PMID: 7024999 Review.
Cited by
- Cilia Distal Domain: Diversity in Evolutionarily Conserved Structures.
Soares H, Carmona B, Nolasco S, Viseu Melo L, Gonçalves J. Soares H, et al. Cells. 2019 Feb 14;8(2):160. doi: 10.3390/cells8020160. Cells. 2019. PMID: 30769894 Free PMC article. Review. - Proteomic analysis of isolated ciliary transition zones reveals the presence of ESCRT proteins.
Diener DR, Lupetti P, Rosenbaum JL. Diener DR, et al. Curr Biol. 2015 Feb 2;25(3):379-384. doi: 10.1016/j.cub.2014.11.066. Epub 2015 Jan 8. Curr Biol. 2015. PMID: 25578910 Free PMC article. - Mesotrypsin and caspase-14 participate in prosaposin processing: potential relevance to epidermal permeability barrier formation.
Yamamoto-Tanaka M, Motoyama A, Miyai M, Matsunaga Y, Matsuda J, Tsuboi R, Hibino T. Yamamoto-Tanaka M, et al. J Biol Chem. 2014 Jul 18;289(29):20026-38. doi: 10.1074/jbc.M113.543421. Epub 2014 May 28. J Biol Chem. 2014. PMID: 24872419 Free PMC article. - Multiple pathways are involved in DNA degradation during keratinocyte terminal differentiation.
Yamamoto-Tanaka M, Makino T, Motoyama A, Miyai M, Tsuboi R, Hibino T. Yamamoto-Tanaka M, et al. Cell Death Dis. 2014 Apr 17;5(4):e1181. doi: 10.1038/cddis.2014.145. Cell Death Dis. 2014. PMID: 24743736 Free PMC article. - Glycosaminoglycans in porcine lung: an ultrastructural study using cupromeronic blue.
Erlinger R. Erlinger R. Cell Tissue Res. 1995 Sep;281(3):473-83. doi: 10.1007/BF00417864. Cell Tissue Res. 1995. PMID: 7553767
References
- J Ultrastruct Res. 1972 Mar;38(5):500-10 - PubMed
- J Cell Biol. 1973 May;57(2):373-87 - PubMed
- Prog Biophys Mol Biol. 1972;25:1-70 - PubMed
- J Protozool. 1974 May;21(2):299-312 - PubMed
- J Cell Sci. 1971 Sep;9(2):435-41 - PubMed