Nosocomial enterococcal blood stream infections in the SCOPE Program: antimicrobial resistance, species occurrence, molecular testing results, and laboratory testing accuracy. SCOPE Hospital Study Group - PubMed (original) (raw)

Nosocomial enterococcal blood stream infections in the SCOPE Program: antimicrobial resistance, species occurrence, molecular testing results, and laboratory testing accuracy. SCOPE Hospital Study Group

R N Jones et al. Diagn Microbiol Infect Dis. 1997 Oct.

Abstract

Characteristics of nosocomial enterococcal blood stream infection (NEBSI) isolates obtained from patients at 41 U.S. hospitals participating in the SCOPE Program were studied. Isolates from 480 episodes of NEBSI were characterized according to species and antimicrobial susceptibility profile. Selected isolates were also identified to species and vancomycin resistance genotype using polymerase chain reaction based methods. Polymerase chain reaction genotyping and ribotyping were used as genetic markers for molecular epidemiologic typing. Enterococci were the third most common cause of nosocomial blood stream infection in this study, accounting for 11.7% of all isolates reported. Enterococcus faecalis was the most common species (59.6%), followed by E. faecium (19.4%). Species identification errors involving E. faecium, E. durans, E. avium, and E. raffinosus were observed. Vancomycin resistance was observed in 36.4% of all participating medical centers and varied from 11.1% of medical centers in the Northwest to 60.9% of medical centers in the Southwest. Vancomycin-resistant enterococci accounted for 20.6% of NEBSI in the Northeast, 11.4% in the Southeast, 11.1% in the Southwest, and 9.5% in the Northwest regions. VanA genotypes predominated in the Northeast and Southwest, whereas vanA and vanB genotypes were equally prevalent in the Northwest and Southeast. Molecular typing studies identified strains that were unique to individual hospitals as well as strains that were prevalent in several different hospitals. NEBSI with vancomycin-resistant enterococci continues to escalate among hospitalized patients in all geographic areas of the USA.

PubMed Disclaimer

Cited by

Surveillance strategies and impact of vancomycin-resistant enterococcal colonization and infection in critically ill patients.
Hendrix CW, Hammond JM, Swoboda SM, Merz WG, Harrington SM, Perl TM, Dick JD, Borschel DM, Halczenko PW, Pelz RK, Rocco LE, Conway JE, Brower RG, Lipsett PA. Hendrix CW, et al. Ann Surg. 2001 Feb;233(2):259-65. doi: 10.1097/00000658-200102000-00016. Ann Surg. 2001. PMID: 11176133 Free PMC article.
Role of global surveillance in combating bacterial resistance.
Marchese A, Schito GC. Marchese A, et al. Drugs. 2001;61(2):167-73. doi: 10.2165/00003495-200161020-00002. Drugs. 2001. PMID: 11270935
Application of Next-Generation Sequencing for Characterization of Surveillance and Clinical Trial Isolates: Analysis of the Distribution of β-lactamase Resistance Genes and Lineage Background in the United States.
Mendes RE, Jones RN, Woosley LN, Cattoir V, Castanheira M. Mendes RE, et al. Open Forum Infect Dis. 2019 Mar 15;6(Suppl 1):S69-S78. doi: 10.1093/ofid/ofz004. eCollection 2019 Mar. Open Forum Infect Dis. 2019. PMID: 30895217 Free PMC article.
Pilot study to evaluate 3 hygiene protocols on the reduction of bacterial load on the hands of veterinary staff performing routine equine physical examinations.
Traub-Dargatz JL, Weese JS, Rousseau JD, Dunowska M, Morley PS, Dargatz DA. Traub-Dargatz JL, et al. Can Vet J. 2006 Jul;47(7):671-6. Can Vet J. 2006. PMID: 16898109 Free PMC article.
Classification and identification of enterococci: a comparative phenotypic, genotypic, and vibrational spectroscopic study.
Kirschner C, Maquelin K, Pina P, Ngo Thi NA, Choo-Smith LP, Sockalingum GD, Sandt C, Ami D, Orsini F, Doglia SM, Allouch P, Mainfait M, Puppels GJ, Naumann D. Kirschner C, et al. J Clin Microbiol. 2001 May;39(5):1763-70. doi: 10.1128/JCM.39.5.1763-1770.2001. J Clin Microbiol. 2001. PMID: 11325987 Free PMC article.

Nosocomial enterococcal blood stream infections in the SCOPE Program: antimicrobial resistance, species occurrence, molecular testing results, and laboratory testing accuracy. SCOPE Hospital Study Group - PubMed (original) (raw)