Transferable, plasmid-mediated vanB-type glycopeptide resistance in Enterococcus faecium - PubMed (original) (raw)

Transferable, plasmid-mediated vanB-type glycopeptide resistance in Enterococcus faecium

L B Rice et al. Antimicrob Agents Chemother. 1998 Apr.

Abstract

An approximately 60-kb transferable, vanB-carrying plasmid has been identified in a clinical Enterococcus faecium strain. A similar plasmid has been observed in an unrelated E. faecium strain, suggesting that plasmid transfer of vanB operons occurs in nature and plays a role in the dissemination of VanB-type resistance among strains of E. faecium.

PubMed Disclaimer

Figures

FIG. 1

FIG. 1

Comparison of _Hin_cII digestions of plasmid preparations from GE-1(pLRM19) and GE-1(pLRM20). Lane 1, bacteriophage lambda digested with _Hin_dIII (size standard—sizes denoted on left side of figure); lane 2, GE-1(pLRM20); lane 3, GE-1(pLRM19); lane 4, High-molecular-weight size standards (Bethesda Research Laboratories, Gaithersburg, Md.) (relevant sizes denoted on right side of figure); lanes 5 and 6, Southern transfer of digests at left hybridized with 2.1-kb _Eco_RV fragment internal to the vanHBBXB operon; lanes 7 and 8, Southern transfer of digests at left hybridized with 1.8-kb _Acc_I fragment of pLRM19 (see text).

References

    1. Bonafede M E, Carias L L, Rice L B. Enterococcal transposon Tn5384: evolution of a composite transposon through cointegration of enterococcal and staphylococcal plasmids. Antimicrob Agents Chemother. 1997;41:1854–1858. - PMC - PubMed
    1. Boyce J M, Opal S M, Chow J W, Zervos M J, Potter-Bynoe G, Sherman C B, Romulo R L, Fortna S, Medeiros A A. Outbreak of multidrug-resistant Enterococcus faecium with transferable vanB class vancomycin resistance. J Clin Microbiol. 1994;32:1148–1153. - PMC - PubMed
    1. Centers for Disease Control and Prevention. Nosocomial enterococci resistant to vancomycin—United States, 1989–1993. Morbid Mortal Weekly Rep. 1993;42:597–599. - PubMed
    1. Christie P J, Korman R Z, Zahler S A, Adsit J C, Dunny G M. Two conjugation systems associated with plasmid pCF10: identification of a conjugative transposon that transfers between Streptococcus faecalis and Bacillus subtilis. J Bacteriol. 1987;169:2529–2536. - PMC - PubMed
    1. Clewell D B. Plasmids, drug resistance and gene transfer in genus Streptococcus. Microbiol Rev. 1981;45:409–436. - PMC - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources