Molecular Comparison of... : Journal of Infectious Diseases (original) (raw)

Molecular Comparison of Extraintestinal Escherichia coli Isolates of the Same Electrophoretic Lineages from Humans and Domestic Animals

James R. Johnson
Parissa Delavari
Adam L. Stell
Thomas S. Whittam
Ulrike Carlino
Thomas A. Russo

Journal of Infectious Diseases

183(1):p 154-159, January 1, 2001.

| DOI: 10.1086/317662

Molecular typing methods were used to characterize 38 Escherichia colistrains that originally were isolated from extraintestinal infections and represented 5 multilocus enzyme electrophoretic types (ETs) recovered from both humans and animals. Within each ET, the human and animal isolates did not consistently segregate by host group, according to individual virulence factors (VFs), composite VF-serotype profiles, or pulsed-field gel electrophoresis profiles. Several close matches with respect to VF-serotype profiles were identified between human and canine isolates from different locales. One canine and 2 human isolates of serogroup O6 closely resembled archetypal human pyelonephritis isolate 536 (O6:K15:H31), according to papA sequence and VF-serotype profile. These findings support the hypothesis that certain pathogenic lineages of E. coli cause disease in both humans and animals and that humans may acquire pathogenic E. coli from domestic pets.